{"product_id":"ast-disc-voriconazole-vo-1-g-microbiology-testing-disc-lab-use-only","title":"Liofilchem Voriconazole VO 1µg antifungal susceptibility test - Microbiology test 9168","description":"\u003ch2\u003eVoriconazole VO 1µg  - Antifungal Disc\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003eInstructions For Use\u003c\/em\u003e\u003c\/p\u003e\n\n\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003eVoriconazole VO 1µg Antifungal Discs are high-quality paper discs impregnated with a standardised concentration of antifungal agent, designed for the in vitro susceptibility testing of yeasts by the CLSI disk diffusion method (CLSI Guideline M44, 3rd ed.). The range covers 18 antifungal agents including azoles, polyenes, echinocandins, and antimetabolites, providing comprehensive coverage for clinical and research mycology laboratories. Each disc is manufactured in compliance with WHO and FDA specifications and undergoes rigorous quality control using CLSI reference strains (\u003cem\u003eCandida albicans\u003c\/em\u003e ATCC 90028, \u003cem\u003eCandida parapsilosis\u003c\/em\u003e ATCC 22019, \u003cem\u003eCandida tropicalis\u003c\/em\u003e ATCC 750, and \u003cem\u003eCandida krusei\u003c\/em\u003e ATCC 6258). Inhibition zone diameters are interpreted against validated CLSI breakpoints to classify isolates as Susceptible, Susceptible-Dose Dependent, Intermediate, or Resistant.\u003c\/p\u003e\n\n\u003ch3\u003eTest Procedure\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eAllow discs to equilibrate to room temperature before opening the container (cartridge or canister) to minimise condensation on the discs, which could affect long-term stability\u003c\/li\u003e\n  \u003cli\u003ePrepare a suspension of the test organism to the density of a 0.5 McFarland turbidity standard for \u003cem\u003eCandida\u003c\/em\u003e spp. and 1 McFarland for \u003cem\u003eC. neoformans\u003c\/em\u003e\n\u003c\/li\u003e\n  \u003cli\u003eImmerse a sterile swab in the suspension and squeeze it against the wall of the tube to remove excess liquid\u003c\/li\u003e\n  \u003cli\u003eDrag the swab across the surface of the susceptibility test medium for yeasts (Mueller Hinton Agar + Glucose + Methylene Blue)\u003c\/li\u003e\n  \u003cli\u003ePosition the antifungal discs within 15 minutes of inoculating the plates, pressing firmly onto the agar surface with sterile pliers\u003c\/li\u003e\n  \u003cli\u003eIncubate the plate within 15 minutes of depositing the discs at 35 ± 2°C for 24 hours\u003c\/li\u003e\n  \u003cli\u003eRead at 48 hours only when insufficient growth is observed after 24 hours of incubation\u003c\/li\u003e\n  \u003cli\u003eAt the end of the incubation period, measure the inhibition halos and compare them with the standard CLSI inhibition halo diameters\u003c\/li\u003e\n  \u003cli\u003ePinpoint microcolonies at the zone edge or large colonies within a zone are encountered frequently and should be ignored\u003c\/li\u003e\n  \u003cli\u003eIn the case of mixed colonies, strains must be purified on blood medium or Sabouraud Dextrose Agar before being swabbed on the susceptibility test plates\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eStorage (unopened):\u003c\/strong\u003e −20°C to +8°C until expiry date. Some products must be stored at −20°C maximum — refer to product label\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eStorage (opened cartridge):\u003c\/strong\u003e 2–8°C for no more than 7 days. Return cartridge to its desiccant envelope and resealable bag\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eStorage (opened canister):\u003c\/strong\u003e Label storage temperature for up to 2 months from first opening\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAvailable formats:\u003c\/strong\u003e 1 cartridge × 50 discs (add \/1 to catalogue reference); 5 cartridges × 50 discs — 250 discs total (standard pack)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eIntended use:\u003c\/strong\u003e For professional in vitro diagnostic use only. Not for human or veterinary therapeutic use\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eQuality control strains:\u003c\/strong\u003e \u003cem\u003eCandida albicans\u003c\/em\u003e ATCC 90028; \u003cem\u003eCandida parapsilosis\u003c\/em\u003e ATCC 22019; \u003cem\u003eCandida tropicalis\u003c\/em\u003e ATCC 750; \u003cem\u003eCandida krusei\u003c\/em\u003e ATCC 6258\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eStandards:\u003c\/strong\u003e CLSI Guideline M44 (3rd ed., 2018); CLSI Supplement M60 (2nd ed., 2020)\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Liofilchem","offers":[{"title":"5 cartridges × 50 discs\/cartridge","offer_id":50018134786334,"sku":"KB749168","price":145.0,"currency_code":"CAD","in_stock":true},{"title":"1 cartridge × 50 discs\/cartridge","offer_id":50018134819102,"sku":"KB7491681","price":87.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0936\/5834\/9854\/files\/levonadifloxacin-lnd-10-g-microbiology-ast-disc-microbiology-liofilchem-1235093235.png?v=1777733773","url":"https:\/\/www.kormay.ca\/products\/ast-disc-voriconazole-vo-1-g-microbiology-testing-disc-lab-use-only","provider":"Kormay Biomedicals store","version":"1.0","type":"link"}