{"product_id":"brain-heart-infusion-broth-for-microbiology-lab-testing","title":"Liofilchem Brain Heart Infusion Broth Culture Media 26104","description":"\u003ch2\u003e© Liofilchem® - Brain Heart Infusion Broth - Rev.1 \/ 15.05.2020\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003eInstructions For Use\u003c\/em\u003e\u003c\/p\u003e\n\u003ch3\u003eDescription\u003c\/h3\u003e\n\u003cp\u003eBrain Heart Infusion Broth is a liquid medium used for the cultivation of fastidious and nonfastidious microorganisms, including aerobic and anaerobic bacteria, from clinical specimens, food and environmental samples. This medium is especially suited for the cultivation of coagulase-positive staphylococci for the plasma coagulase test according to ISO 6888. Brain Heart Infusion Broth is recommended by the APHA for examination of water and wastewater and by the CLSI for preparing inocula used in antimicrobial susceptibility tests.\u003c\/p\u003e\n\u003ch3\u003ePreparation\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eDehydrated medium Suspend 37 g of the powder in 1 liter of distilled or deionized water\u003c\/li\u003e\n\u003cli\u003eHeat to boil shaking frequently until completely dissolved\u003c\/li\u003e\n\u003cli\u003eDistribute into final containers\u003c\/li\u003e\n\u003cli\u003eSterilize in autoclave at 121°C for 15 minutes\u003c\/li\u003e\n\u003cli\u003eTEST PROCEDURE If material is being cultured directly from a swab, insert the swab into the broth after inoculation of plated media\u003c\/li\u003e\n\u003cli\u003eFor liquid specimens, transfer a loopful of the specimen into the broth medium using a sterile loop or aseptically pipette the specimen onto plated medium and into the broth\u003c\/li\u003e\n\u003cli\u003eExamine for growth after 24-72 hours of incubation\u003c\/li\u003e\n\u003cli\u003eIt is recommended that liquid media for anaerobic incubation should be reduced prior to inoculation by placing tubes (with loosened caps) under anaerobic conditions for 18-24 hours\u003c\/li\u003e\n\u003cli\u003eAlternatively, the media may be reduced by bringing the media up to 100°C in a boiling waterbath\u003c\/li\u003e\n\u003cli\u003eLoosen screw caps slightly before heating, and tighten during cooling to room temperature\u003c\/li\u003e\n\u003cli\u003eTo perform plasma coagulase tests, according to ISO 6888, inoculate tubes of Brain Heart Infusion Broth with selected colony from Baird Parker Agar plates (ref\u003c\/li\u003e\n\u003cli\u003eIncubate at 37 ± 1°C for 24 ± 2 hours\u003c\/li\u003e\n\u003cli\u003eAdd 0.1 ml of each culture to 0.3 ml of the rabbit plasma\u003c\/li\u003e\n\u003cli\u003eExamine after 4-6 hours incubation at 37°C for clotting of the plasma\u003c\/li\u003e\n\u003cli\u003eINTERPRETING RESULTS Turbidity indicates microbial growth\u003c\/li\u003e\n\u003cli\u003eThe coagulase test is considered positive if the clot volume is more than half of the original liquid volume.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eTechnique\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAPHA (2012): Standard Methods for the Examination of Water\u003c\/li\u003e\n\u003cli\u003eAmerican Public Health Association, American Water Works Association, Water Environment Federation, Washington, D.C\u003c\/li\u003e\n\u003cli\u003eQuality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - Third Edition\u003c\/li\u003e\n\u003cli\u003eCLSI document M22-A3\u003c\/li\u003e\n\u003cli\u003eWayne, PA: Clinical and Laboratory Standards Institute, 2004\u003c\/li\u003e\n\u003cli\u003eISO 6888-3:2003\u003c\/li\u003e\n\u003cli\u003eMicrobiology of food and animal feeding stuffs − Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) −Detection and MPN\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e 2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShelf life:\u003c\/strong\u003e Medio deshidratado: 4 años\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Liofilchem","offers":[{"title":"100\/pack","offer_id":50019560849694,"sku":"KB7426104","price":611.0,"currency_code":"CAD","in_stock":true},{"title":"10 mL x 20","offer_id":50019560882462,"sku":"KB7424104","price":107.0,"currency_code":"CAD","in_stock":true},{"title":"2 mL x 20","offer_id":50019560915230,"sku":"KB7424141","price":115.0,"currency_code":"CAD","in_stock":true},{"title":"200 mL x 6","offer_id":50019560947998,"sku":"KB74412010","price":191.0,"currency_code":"CAD","in_stock":true},{"title":"5 mL x 50","offer_id":50019560980766,"sku":"KB7427502","price":262.0,"currency_code":"CAD","in_stock":true},{"title":"9 mL x20","offer_id":50019561013534,"sku":"KB7424480","price":94.0,"currency_code":"CAD","in_stock":true},{"title":"100 g","offer_id":50019561046302,"sku":"KB74620008","price":123.0,"currency_code":"CAD","in_stock":true},{"title":"5 Kg","offer_id":50019561079070,"sku":"KB746100085","price":3676.0,"currency_code":"CAD","in_stock":true},{"title":"500 g","offer_id":50019561111838,"sku":"KB74610008","price":441.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0936\/5834\/9854\/files\/brain-heart-infusion-broth-for-microbiology-lab-testing-microbiology-liofilchem-1235093718.jpg?v=1777715231","url":"https:\/\/www.kormay.ca\/products\/brain-heart-infusion-broth-for-microbiology-lab-testing","provider":"Kormay Biomedicals store","version":"1.0","type":"link"}