Description
Bi-plate for detection of resistance to carbapenems in Enterobacteriaceae, including OXA-48 strains, directly from clinical specimens.
ChromaticTM CRE / ChromaticTM OXA-48 is a ready-to-use plate containing two distinct selective and chromogenic media used for the isolation and identification of carbapenemase-producing Enterobacteriaceae including the Carbapenem-hydrolysing oxacillinase-48.
Carbapenems are the last shelter from multi-resistant Gram-negative bacterial infections. Carbapenemase is an enzyme class which can be produced by Klebsiella pneumoniae and by other organisms including Serratia and Enterobacter spp, as well as Escherichia coli and Citrobacter freundii.
OXA-48-type carbapenem-hydrolysing class D β-lactamases are increasingly reported in enterobacterial species. These enzymes hydrolyse penicillins at a high level and carbapenems at a low level, posses poor activity against broad-spectrum cephalosporins and are not susceptible to β-lactamase inhibitors. Their frequent association with ESBL and permeability defects increase the level of resistance to carbapenems.
Patients infected by carbapenem-resistant Enterobacteriaceae often received inadequate empirical therapy until the pathogen’s resistance is recognized. In addition, the OXA-48-type producers with low MICs, categorized as susceptible to carbapenems by the EUCAST and CLSI guidelines, are very difficult to detect and may spread unnoticed, evolving then to resistance phenotypes. Therefore, selective isolation and sensitive detection of these
pathogens are essential to limit their spread.