An auditor should be able to reconstruct why a laboratory selected a product, how it was controlled, and how the final result was reviewed. This guide uses the manufacturer IFU for catalogue 610098 as its technical anchor and translates it into practical review questions for professional laboratories.
Start with the product’s documented role
ALKALINE PEPTONE WATER is recommended by OMS and APHA as a tranport and enrichment medium for Vibrio spp. The enrichment in liquid medium is necessary for isolation of Vibrio from feces (after the first 48 hours of disease), for the control of carriers and for the examination of drinks , foods or sewage. The high value of pH slows down the development of common contaminants, so that after 6-7 hours of incubation, vibrio, if present, stand out on microscopical examination in dark field.
Review Liofilchem Alkaline Peptone Water culture media 610098 at Kormay Biomedicals
Design the workflow before opening the pack
Define the sample type, inoculum or preparation step, incubation conditions, control organisms, reading window, confirmation pathway, and acceptance criteria in the SOP. Record the catalogue number, lot, expiry, analyst, equipment, and any deviation that could change interpretation.
Procedure points worth controlling
The medium is inoculated with few drops or a loopful of material and incubated at 36 ± 1°C. After 12-24 hours, inoculate a loopful of the culture broth on a isolation medium. For confirming the diagnosis, submit the suspected colonies to serological and biochemical identification tests.
Storage and handling
The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared media at 2-8 °C.
A practical pre-release checklist
- Current IFU revision and catalogue code verified
- Receipt, storage, lot, and expiry documented
- Controls and acceptance criteria defined before testing
- Reading time and observations recorded without forcing an ambiguous endpoint
- Confirmation and deviation actions linked to the laboratory SOP
Final perspective
Useful technical content should help a laboratory make a better-controlled decision, not replace the IFU or validated method. Confirm the current manufacturer documentation and local regulatory requirements before implementation.