The strongest purchasing decision connects the catalogue item to the method, documentation, storage capacity, and expected workload. This guide uses the manufacturer IFU for catalogue 610377 as its technical anchor and translates it into practical review questions for professional laboratories.
Start with the product’s documented role
Alkaline Saline Peptone Water ISO (ASPW) is a liquid medium used for the selective enrichment of Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus from food, animal feed and environmental samples. This medium complies to ISO 21872-1.
Review Liofilchem Alkaline Saline Peptone Water ISO 610377 at Kormay Biomedicals
Design the workflow before opening the pack
Define the sample type, inoculum or preparation step, incubation conditions, control organisms, reading window, confirmation pathway, and acceptance criteria in the SOP. Record the catalogue number, lot, expiry, analyst, equipment, and any deviation that could change interpretation.
Procedure points worth controlling
Following the procedure described by ISO 21872-1 1.Take test portions (25 g or 25 ml) and homogenize in a bottle containing 225 ml of ASPW. 2.Incubate at 41.5 ± 1°C and/or 37 ± 1°C for 6 ± 1 h (a). 3.Transfer 1 ml of the primary enrichment culture into a tube containing 10 ml of ASPW (b). 4.Incubate at 41.5 ± 1°C (V. parahaemolyticus and V. cholerae) and/or 37 ± 1°C (V. vulnificus) for 18 ± 1 h (c). 5.From the cultures obtained in the ASPW, inoculate with 1 µl sampling loop the surface of a TCBS agar plate (ref. 11195) to obtain well-isolated colonies (for more details see the IFU of TCBS agar).
Read and document the result
Growth in ASPW is indicated by turbidity compared to an uninoculated control. For interpreting the test results on TCBS agar see the relevant IFU.
Storage and handling
The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store tubes and bottles at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
Safety and professional-use reminder
The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for professional use only and must be used by properly trained operators.
A practical pre-release checklist
- Current IFU revision and catalogue code verified
- Receipt, storage, lot, and expiry documented
- Controls and acceptance criteria defined before testing
- Reading time and observations recorded without forcing an ambiguous endpoint
- Confirmation and deviation actions linked to the laboratory SOP
Final perspective
Useful technical content should help a laboratory make a better-controlled decision, not replace the IFU or validated method. Confirm the current manufacturer documentation and local regulatory requirements before implementation.