Product performance begins before the package reaches the bench, making receipt and storage part of the analytical workflow. This guide uses the manufacturer IFU for catalogue 10406 as its technical anchor and translates it into practical review questions for professional laboratories.
Start with the product’s documented role
Aeromonas Agar is a selective medium used for the detection and enumeration of Aeromonas spp in food, water and other materials of sanitary importance.
Review Liofilchem AEROMONAS Agar Culture Media 10406 at Kormay Biomedicals
Design the workflow before opening the pack
Define the sample type, inoculum or preparation step, incubation conditions, control organisms, reading window, confirmation pathway, and acceptance criteria in the SOP. Record the catalogue number, lot, expiry, analyst, equipment, and any deviation that could change interpretation.
Procedure points worth controlling
Inoculate the medium by either spread plating or membrane filtration
Read and document the result
Examine the plates for the presence of dark green, opaque colonies with darker centre (colony size 0.5 to 1.5 mm in diameter). Confirm by subculturing to a non selective agar medium looking for oxidase reaction (ref. 88029) and trehalose fermentation (ref. 88219). Any presumptive colony that is oxidase-positive and ferments trehalose is considered to be Aeromonas spp.
Quality control as a decision tool
Plates are inoculated with the microbial strains indicated in the QC table. Inoculum for productivity: 50-100 CFU. Inoculum for selectivity: 104-106 CFU. Incubation conditions: 18-24 h at 30-35°C . QC Table. Microorganism Specification Aeromonas hydrophila ATCC 7966 Good growth, opaque green colonies with a dark centre Escherichia coli ATCC 25922 Inhibition Enterococcus faecalis ATCC 19433 Inhibition WARNING AND
Storage and handling
Store prepared plates at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
Safety and professional-use reminder
The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for professional use only and must be used by properly trained operators.
A practical pre-release checklist
- Current IFU revision and catalogue code verified
- Receipt, storage, lot, and expiry documented
- Controls and acceptance criteria defined before testing
- Reading time and observations recorded without forcing an ambiguous endpoint
- Confirmation and deviation actions linked to the laboratory SOP
Final perspective
Useful technical content should help a laboratory make a better-controlled decision, not replace the IFU or validated method. Confirm the current manufacturer documentation and local regulatory requirements before implementation.