Product and use case
Liofilchem TRYPTONE SULFITE NEOMYCIN Agar Culture Media 610074 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is tryptone Sulfite Neomycin (TSN) Agar is a medium used for the selective isolation of sulfite reducing-clostridia, especially Clostridium perfringens, from food and other materials.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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When this format is useful
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
What to read after incubation
While the growth of other sulfite-reducing clostridia is almost completely inhibited at 46°C, C. perfringens form black colonies. The plates should be inspected immediately after opening, otherwise the black colonies become paler in color due to oxidation of iron sulfide.
Selective or enrichment logic
Tryptone (Pancreatic Digest of Casein) provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Yeast extract is a source of vitamins, particularly of B-group. Sodium sulfite and ferric citrate are H2S indicators. Neomycin and Polymyxin suppress largely the accompanying bacterial flora. Agar is the solidifying agent.
Sample handling workflow
- Preparation: Dehydrated medium Suspend 41.6 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. Sterilize in autoclave at 115°C for 20 minutes. DO NOT OVERHEAT. The culture medium should be prepared and used the same day.
- Procedure: Inoculate tubes or plates of Tryptone Sulfite Neomycin Agar by stabbing deep tubes or streaking plates with the test sample. Incubate for 18-24 hours at 46 ± 1°C in anaerobic atmosphere.
Technical verification points
- Formula / composition: (g/l) Tryptone 15.0 Yeast Extract 10.0 Sodium Sulfite 1.0 Ammonium Ferric Citrate 0.5 Neomycin Sulfate 0.05 Polymyxin B Sulfate 0.02 Agar 15.0 Final pH 7.2 ± 0.2 at 25°C
- Quality control: The medium is inoculated with the microbial strains indicated in the QC table. Inoculum for productivity: 10-100 CFU. Inoculum for selectivity: 104-106 CFU. Incubation conditions: anaerobically at 46 ± 1°C for 18-24 hours. QC Table. Microorganism Growth Black Colonies Clostridium perfringensATCC 13124 Good + Escherichia coli ATCC 25922 Inhibited --- Pseudomonas aeruginosaATCC 27853 Inhibited --- WARNING AND
Storage, hazards, and disposal
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for professional use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store tubes at 2-8°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610074
- Catalog/document code matched: 610074, 2015
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/tryptone-sulfite-neomycin-agar-for-microbiology-lab-testing-1