Laboratory use profile
Liofilchem MUELLER HINTON II Agar Culture Media 610627 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is medium recommended for antimicrobial susceptibility testing of common, rapidly growing aerobic microorganisms by the disc diffusion
This product belongs in antimicrobial or antifungal susceptibility testing, where the value is not the disc or strip alone but the controlled relationship between organism identity, inoculum, medium, incubation, and interpretation.
View this product on Kormay Biomedicals
Use case and sample types
In susceptibility testing, the consumable is only useful when the inoculum, medium, incubation, reading endpoint, and interpretive system are controlled together. The lab should treat the result as a method output, not as a standalone product property.
Decision criteria after incubation
At the end of the incubation period, the inhibition zone diameters are measured and interpreted according to the current breakpoints and QC criteria published by CLSI or EUCAST. For enterococci with vancomycin, incubation time shall be increased to 24 h. Report the organism as susceptible, intermediate or resistant to the agents that have been tested.
Method logic
Acid hydrolysate of casein and beef extract provide amino acids, nitrogen, minerals, vitamins, carbon and other nutrients which support the growth of microorganisms. Starch acts as a protective colloid against toxic molecules which can be present in the medium. Hydrolysis of starch during autoclaving supplies a little amount of glucose, which is a source of energy. Agar is the solidifying agent. The Kirby-Bauer method is based on the diffusion, through the agar, of the antimicrobial substance which soaks the paper disc. Each disc has a single concentration of the antimicrobial agent that inhibits the microorganism growth showing a halo around the disc. The diameter of the inhibiting halo is correlated with the Minimal Inhibitory Concentration (MIC).
Preparation and execution notes
- Preparation: Dehydrated medium Suspend 38.0 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50°C. Aseptically dispense in Petri dishes. Medium in bottles Melt the content of the tube/bottle in a water bath at 100°C (loosing the cap partially removed) until completely dissolved. Then screw the cap and check the homogeneity of the dissolved medium, if it is the case turning the bottle upside down. Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into Petri dishes.
- Materials required: Standard microbiological supplies and equipment such as: Autoclave, test tubes, inoculating loops, swabs, incubator,
- Procedure: Ensure there is no visible moisture on the plates before use. Prepare a standardized suspension 0.5 McFarland of the test organism using either the colony suspension or broth culture method. Dip a sterile cotton swab into the adjusted suspension. Inoculate the surface of the plate by streaking the swab over the entire agar surface. Apply the antimicrobial discs onto the surface of the inoculated agar plate. Incubate aerobically plates in an inverted position at 35 ± 2°C for 16-18 hours (CLSI) or 35 ± 1°C for 18 ± 2h (EUCAST). For more detailed instructions, please refer to the current CLSI and/or EUCAST recommendations. Note: • Do not prolong incubation beyond the recommended period as it will affect zone sizes and invalidate interpretative criteria. • It is recommended to use the inoculum suspension within 15 minutes of
Formula, QC, and performance
- Formula / composition: * (g/l) Beef Extract 2.0 Acid Hydrolysate of Casein 17.5 Starch 1.5 Agar 17.0 Final pH 7.3 ± 0.2 at 25°C *Adjusted and/or supplemented as required with appropriate salts to provide the following concentrations of ions and meet
- Quality control: organisms. SPECIMENS Mueller Hinton II Agar is used for AST of pure cultures that have been isolated from clinical specimens. Specimens should be obtained before antimicrobial therapy (where possible) and promptly delivered to the laboratory for examination. Clinical samples are not inoculated directly onto MH II Agar. © Liofilchem - Mueller Hinton II Agar - Rev.0 / 23.07.2024 Page 2 of 10 The microorganism to be tested must first be isolated on a nonselective solid medium, such as blood agar or tryptic soy agar (TSA). Refer to specific guidelines for more detailed information.
- Performance: specifications according to ISO/TS 16782: 20-25 mg/l of calcium, 10-12.5 mg/l of magnesium, manganese and zinc below 8 mg/l and 3 mg/l, respectively.
Risk controls for routine use
- Limitations: Invalid results can be caused by poor specimen quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the principles of the procedures, including its
- Warnings / precautions: 1) For in vitro diagnostic use (IVD). 2) For laboratory professional use only. 3) Operators must be trained and have certain experience. Please read the instructions carefully before using the product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. 4) Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. 5) Do not use if the product or packaging appears to be damaged. 6) Follow standard
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store bottles and prepared plates at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610627
- Catalog/document code matched: 610627, 2024
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/mueller-hinton-ii-agar-for-microbiology-lab-testing-1