Product overview
Liofilchem Selenite Cystine Broth ISO 6579 Culture Media 24485 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is selenite Cystine Broth (SC) is a medium used for the selective enrichment of Salmonella spp from food, including milk and dairy products, animal feedstuffs and environmental samples from the area of food production and handling. This medium complies with the recommendations of ISO 6579-1 which describes its usage for the detection of Salmonella Typhi and Paratyphi.
This product is relevant to food safety and environmental monitoring programs where Salmonella screening is part of raw material, finished product, or hygiene verification testing.
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Where this product fits
This product fits screening programs where a negative or presumptive result can affect raw-material release, finished-product hold decisions, or hygiene investigations. The lab should connect the medium to its enrichment sequence, confirmation method, and written rule for when suspect colonies move forward.
Result signals and confirmation
of a red precipitate before inoculation indicates overheating of the medium, in which case the selective properties are significantly reduced.
Method principle
Enzymatic digest of casein provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Lactose is the fermentable carbohydrate. Sodium phosphate is the buffering agent. Sodium selenite inhibits Gram-positive and Gram-negative bacteria other then salmonellae. L-Cystine is a reducing agent incorporated to enhance the recovery capacity of the medium.
Bench workflow
- Preparation: Dehydrated medium Suspend 23.1 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boiling to dissolve completely. Avoid overheating. DO NOT AUTOCLAVE. Note
- Procedure: Refer to appropriate
Controls and performance checks
- Formula / composition: * (g/l) Enzymatic Digest of Casein 5.0 Lactose 4.0 Disodium Hydrogen Phosphate dodecahydrate 10.0 Sodium Hydrogen Selenite 4.0 L-Cystine 0.1 Final pH 7.0 ± 0.2 at 25°C *Adjusted and/or supplemented as required to meet
Safety and documentation points
- Hazards: 2.1 Classification of the su bstance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Acute Tox. 4, Acute toxicity, Oral (Category 4); H302 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H302 Harmful if swallowed. Precautionary statement(s) P270 Do no eat, drink or smoke when using this produ ct. Supplemental Hazard Statements none 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Contro l parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB7424485
- Catalog/document code matched: 6579, 24485, 2018
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/selenite-cystine-broth-iso-6579-for-microbiology-lab-testing