Product and use case
Liofilchem Fluid Thioglycollate Medium Culture Media 610050 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is fluid Thioglycollate Medium is a general purpose liquid enrichment medium used for sterility control of pharmaceutical products and for cultivation and isolation of fastidious anaerobic and aerobic microorganisms. The composition is in accordance with the requirements of the Harmonized US, European and Japanese Pharmacopoeia as well as with ISO 7937 for isolation of Clostridium perfringens.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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When this format is useful
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
What to read after incubation
Turbidity of the medium indicates microbial growth. Obligate anaerobic microorganisms such as Clostridium sporogenes are growing in the lower, yellowish part of the broth medium. The growth of facultative anaerobic microorganisms such as Staphylococcus aureus is distributed throughout all the medium. Aerobic microorganisms such as Pseudomonas aeruginosa are able to grow in the upper slightly pink layer (oxidized part) of the medium.
Selective or enrichment logic
Enzymatic digest of casein provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Yeast extract is a source of vitamins, particularly of B-group. Glucose is a source of energy. Sodium chloride maintains the osmotic balance of the medium. Sodium thioglycollate and L-cystine are included to reduce the redox potential of the medium and create an anaerobic atmosphere. These reducing agents also neutralize the bacteriostatic effects of mercury and other heavy metal compounds in the
Sample handling workflow
- Preparation: to be tested for sterility. Resazurin is an oxidation-reduction indicator being pink when oxidized ad colorless when reduced. The small amount of agar assists in the maintenance of a low redox potential by stabilizing the medium against convection currents, thereby maintaining anaerobiosis in the lower depths of the medium.
- Procedure: The medium can be directly inoculated with the test sample (the amount of the inoculated sample material should not be exceed 10% volume of the medium). Incubate at 30-35°C for up to 14 days. Growth of strictly aerobic bacteria can be improved by slightly loosening the cap. According to ISO 7937 for confirmation of Clostridium perfringens incoculate each black colony from Sulfite Cycloserine Agar (ref. 402700) into Fluid Thioglycollate Medium. Incubate at 37 ± 1°C for 18-24 hours. Subsequently, transfer 5 drops of the enrichment culture into Lactose Sulfite Medium (ref. 610358) and incubate at 46 ± 1°C for 18-24 hours.
Technical verification points
- Performance: specifications; Grams per litre of purified water.
Storage, hazards, and disposal
- Warnings / precautions: For in-vitro diagnostic use (see the product list the next page). For professional use only. Operators must be trained and have certain experience in the laboratory methods. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store bottles and tubes at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Skin Sens.1 , Skin sensibilization (Category 1); H317 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H317 May cause an allergic skin reaction.
- Exposure controls: 8.1 Control parameters • DNEL/DMEL o Sodium thioglycolate ; CAS No. : 367-51-1 Limit value type : DNEL Consumer (systemic) ▪ Exposure route : Oral ▪ Exposure frequency : Long-term ▪ Limit value : 0.174 mg/m3 ▪ Exposure route : Dermal ▪ Exposure frequency : Long-term ▪ Limit value : 19.3 ug/kg bw/day SAFETY DATA SHEET (According to Regulation EC n° 1907/2006 and to Commission Regulation EU 2020/878) Revision: 6 / Revision Date: 11.03.2026 ©Liofilchem s.r.l.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610050
- Catalog/document code matched: 610050, 2022, 7937
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/fluid-thioglycollate-medium-for-microbiology-lab-testing