Product and use case
Liofilchem PFIZER Selective ENTEROCOCCUS Agar Culture Media 610319 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is pFIZER SELECTIVE ENTEROCOCCUS AGAR is a medium in powder used for the enumeration of fecal streptococci by membrane filtration method.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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When this format is useful
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
What to read after incubation
On the agar surface count all the brown-black colonies. Express the result as UFC (Units Forming Colonies) / 100 ml.
Selective or enrichment logic
Peptone, tryptone and yeast extract provide nitrogen and other nutrients necessary to support the bacterial growth. Bacteriological bile inhibits Gram-positive bacteria other than enterococci. Organisms positive for esculin hydrolysis hydrolyze the glycoside esculin to esculetin and dextrose. The esculetin reacts with the ferric citrate to form a dark brown or black complex. Sodium chloride keeps the osmotic balance of the medium. Sodium azide inhibits the bacterial flora grown during the preliminary test, except for enterococci. Sodium citrate is the buffer of the medium. Agar is the solidifying agent.
Sample handling workflow
- Preparation: Suspend 58.0 g of powder into 1 liter of distilled or deionized water. Heat until completely dissolved. Autoclave at 121°C for 15 minutes. Dispense in petri dishes.
- Procedure: Membrane Filtration Method Filter the suspension of the sample through a sterile membrane to give 20-200 colonies on the membrane surface. Transfer the membrane on the agar surface, invert the plates and incubate at 36+/-1°C for 18-48 hours.
Technical verification points
- Formula / composition: (g/l) Peptone 3.3 Yeast extract 5.35 Tryptone 17.0 Bacteriological Bile 10.0 Esculine Hydrate 1.0 Ammonium Ferric Citrate 0.5 Sodium Chloride 5.0 Sodium azide 0.25 Sodium Citrate 1.0 Agar 14.0 pH finale 7.1 ± 0.2
Storage, hazards, and disposal
- Storage / shelf life: CONDITIONS The powder is very hygroscopic: store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared media at 2-8°C. WARNING AND PRECAUTION The product is not classified as hazardous by current legislation and does not contain harmful substances in concentrations of ≥1%. The product is designed for In vitro diagnostic use and must be used only by properly trained operators.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Acute Tox. 4, Acute toxicity, Oral (Category 4); H302 Aquatic Chronic 3, Chronic aquatic toxicity (Category 3); H412 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H302 H412 Harmful if swallowed. Harmful to aquatic life with long lasting effects.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Component N. CAS Value Control parameters Basis TWA 0,1 mg/m3 Indicative limit values for occupatio nal exposure to chemicals Sodium azide 26628-22-8 STEL 0,3 mg/m3 Indicative limit values for occupationa l exposure to chemicals 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610319
- Catalog/document code matched: 610319
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/pfizer-selective-enterococcus-agar-for-microbiology-lab-testing