Laboratory use profile
Liofilchem TSYE Agar (Tryptic Soy Yeast Extract Agar) Culture Media 610349 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is tryptone Soy Yeast Extract Agar (TSYEA) is a medium used for confirmation of L. monocytogenes or Listeria spp in the food chain. This medium complies with the recommendations of ISO 11290-1 and ISO 11290-2.
This product supports Listeria workflows in ready-to-eat food, dairy, meat, and environmental monitoring programs where early presumptive recovery must lead into confirmation.
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Use case and sample types
Use this product where the lab must protect a recovery step before confirmation. For ready-to-eat food and environmental samples, the important decision is not only whether growth appears, but whether the enrichment or plating step gives the organism enough opportunity to recover from stress, competing flora, preservatives, or cold-chain exposure.
Decision criteria after incubation
Typical colonies of Listeria spp on TSYEA are 1-2 mm in diameter, convex, colorless and opaque with an entire edge. Colonies display a bluish color and a granular surface under obliquely transmitted light. Confirmation tests should be performed. Refer to appropriate
Method logic
Enzymatic digest of casein and papaic digest of soyabean meal provide amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Yeast extract is a source of vitamins, particularly of B-group. Sodium chloride maintains the osmotic balance of the medium. Dipotassium phosphate is the buffering agent. Glucose is the fermentable carbohydrate. Agar is the solidifying agent.
Preparation and execution notes
- Preparation: Dehydrated medium Suspend 49 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. Sterilize in autoclave at 121°C for 15 min. Medium in bottles Melt the content of the bottle in a water bath at 100°C (loosing the cap partially removed) until completely dissolved. Then screw the cap and check the homogeneity of the dissolved medium, if it is the case turning the bottle upside down. Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into Petri dishes.
- Procedure: Inoculate the plates by streaking suspect colonies from selective agars, i.e. O.A. Listeria Agar (ref. 10620), Listeria Palcam Agar (ref. 10041), trying to achieve well isolated colonies. Incubate at 37°C for 18-24 hours under aerobic atmosphere.
Formula, QC, and performance
- Formula / composition: (g/l) Enzymatic Digest of Casein 17.0 Papaic Digest of Soyabean Meal 3.0 Yeast Extract 6.0 Sodium Chloride 5.0 Dipotassium Hydrogen Phosphate 2.5 Glucose 2.5 Agar 13.0 Final pH 7.3 ± 0.2 at 25°C
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The produ ct must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610349
- Catalog/document code matched: 610349, 2018, 11290
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/tsye-agar-tryptic-soy-yeast-extract-agar-unit-500-g