Laboratory use profile
Liofilchem Dextrose Tryptone Broth Culture Media 610376 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is dextrose Tryptone Broth (DTB) is a liquid medium used for the detection and cultivation of different organisms causing spoilage in foods and other products. This medium complies with the recommendations of the American Public Health Association (APHA) for the microbiological examination of low and medium-acid canned foods (pH 4.5 and above).
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Use case and sample types
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Decision criteria after incubation
A colour change of the medium from purple to yellow indicates production of acid by microorganism which ferment dextrose. Plates are streaked from positive tubes to obtain pure isolates. Consult appropriate
Method logic
Tryptone provides nitrogen, vitamins, minerals and amino acids for organisms growth. Dextrose is the fermentable carbohydrate providing carbon and energy. Bromocresol purple is the pH indicator.
Preparation and execution notes
- Procedure: Inoculate 9 ml of the broth with 1-2 g of solid material or 1-2 ml of liquid sample. Incubate duplicate sets of tubes at 35°C for 96-120 hours and at 55°C for 24-48 hours. Check for growth at frequent intervals up to maximum time of incubation. NOTE: It is recommended to perform aerobic cultivation in DTB in parallel with anaerobic cultivation in other media, such as Liver Broth (ref. 610143) or Cooked Meat Medium (ref. 24071).
Formula, QC, and performance
- Performance: specifications; Grams per litre of purified water.
Safety and documentation points
- Hazards: 2.1 Classification of the s ubstance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610376
- Catalog/document code matched: 610376, 2021
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/dextrose-tryptone-broth-for-microbiology-lab-testing