Application snapshot
Liofilchem Lowenstein Jensen Medium Culture Media 610026 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is lOWENSTEIN JENSEN MEDIUM is used for the cultivation of Mycobacteria from both clinical specimen and pure culture.
This product fits laboratories that culture or support workups for mycobacteria, where slow growth, selective recovery, incubation duration, and contamination control all affect the usefulness of the final result.
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Application fit for the lab
Mycobacteria workflows need a medium choice that accounts for slow growth, long incubation, contamination pressure, and careful colony review. The method should define how often plates are examined, what colony features matter, and which follow-up identification steps are required.
Expected colonies or reaction pattern
free flowing, homogeneous. Color: medium to dark green-blue. Prepared medium
How to build it into the SOP
- Procedure: The tubes are inoculated in duplicate, streaking the material to ba examined onto the surface of the medium. Incubate at 36 ± 1 °C for 2 to 12 weeks in a carbon dioxide enriched atmosphere, in darkness, with the caps loosened.
Composition and QC checkpoints
- Formula / composition: (g/600 ml) Magnesium Sulphate 0.24 Asparagine 3.6 Monopotassium Phosphate 2.4 Magnesium Citrate 0.6 Potato Flour 30.0 Malachite Green 0.4 Final pH = 7.0 ± 0.2 at 25 °C. DIRECTIONS Suspend 37.2 g of powder in 600 ml of distilled or deionized water. Add 12 ml of Glycerol supplement (code 80021) and heat until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50 °C and add 1000 ml of a uniform suspension of fresh eggs, collected and suspended under aseptic conditions. Dispense into sterile tubes.
Handling limits and documentation
- Limitations: Negative culture results do not rule out an active mycobacterial infection. Some factors responsible for unsuccessful cultures are: -The specimen was not representative of the infectious material, i.e. saliva instead of sputum. -The mycobacteria were destroyed during digestion and decontamination of the specimen. -Gross contamination interfere with the growth of mycobacteria. -Proper aerobic and increased CO2 conditions were not respected during incubation.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared tubes at 2-8 °C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Eye Irrit.2, Eye irritation (Category 2); H319 Aquatic Chronic 3, Chronic aquatic toxicity (Category 3); H412 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H315 H412 Causes skin irritation Harmful to aquatic life with long lasting effects Precautionary statement(s) P264 Wash hands thoroughly after
- Exposure controls: 8.1 Control parameters Contains no substances with occupational exposure limit value 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non - recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74610026
- Catalog/document code matched: 610026
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/lowenstein-jensen-medium-for-microbiology-lab-testing