A susceptibility result can shift before the organism changes. That is why BURKHOLDERIA cepacia Selective Agar(BCSA) Culture Media must be considered together with inoculum, atmosphere, incubation, and endpoint reading. The discussion below connects its documented role with preparation variables, controls, interpretation, and the limits of inference. Catalogue 11506 is discussed as a laboratory measurement component; implementation should follow the laboratory SOP and validated reference method.
Analytical scope and method role
BURKHOLDERIA CEPACIA SELECTIVE AGAR (BSCA) is a selective medium for the isolation of Burkholderia cepacia from clinical andnon-clinical specimens.
Method principle
The medium contains a variety of proteic substrates derived from enzymatic digestion, inorganic salts and other nutrients which satisfynutritional requirements of these microorganisms. Crystal violet and bile salts inhibit Gram-positive cocci while Ticarcillin, Polymyxin Band Gentamicin inhibit also Gram-negative microorganisms. Alkaline end products from the metabolism of pyruvate raise the pH of themedium causing the color of the indicator (phenol red) to change from light orange to pink-red in the growth area.
Formula and functional interpretation
(g/l)Meat Extract 5.0Yeast Extract 4.0Ammonium Sulfate 1.0Magnesium Sulfate 0.2Ferrous Ammonium Sulfate 0.01Bile Salts 1.5Potassium Dihydrogen Phosphate 4.4Sodium Phospahte 1.4Sodium Pyruvate 7.0Phenol Red 0.02Crystal Violet 0.001Agar 15.0Ticarcillin 0.1Polymyxin B150000 IUGentamicin 0.005Final pH 6.2 ± 0.2
Review the nutritive, selective, differential, buffering, reducing, and indicator components against the expected target and background flora. Preparation errors can change recovery, inhibition, colour development, or endpoint visibility even when incubation is correct.
Preparation and execution: controlled method points
Procedure: Take the sample and, if necessary, dilute 1:2 the sample in Ringer's Solution (Ref. 81059). Streak onto a BURKHOLDERIA CEPACIASELECTIVE AGAR (BSCA) plate and incubate at 36 ± 1°C for 48-72 hours.
Translate the method into controlled worksheet fields: catalogue and lot, preparation mass or volume, water quality, pH where applicable, heating or sterilization, supplements, dispense volume, preparation date, expiry assignment, incubation atmosphere and temperature, reading window, equipment, and analyst. Record departures as deviations instead of silently normalizing them.
Quality control: what the control result protects
1.Control of general characteristics, label and print2.Sterility control7 days at 22 ± 1°C, in aerobiosis7 days at 36 ± 1°C, in aerobiosis3.Microbiological controlInoculum for productivity: 10-100 UFC/mlInoculum for selectivity: 104-105 UFC/mlInoculum for specificity: !104 UFC/mlIncubation Conditions: 48-72 h at 36 ± 1°C, in aerobiosisMicroorganismGrowthFeaturesBurkholderia cepacia ATCC 25608GoodGrey colonies with bright pink medium*Burkholderia cepaciaATCC 24416GoodSage green colonies with bright pink medium*Pseudomonas aeruginosaATCC 27853Inhibited---* low numbers of colonies may not
Challenge both sides of performance. Include a target reaction demonstrating recovery and, when relevant, a non-target or alternate reaction challenging inhibition or differentiation. Record expected and observed responses, acceptance criteria, strain and passage, lot, incubation, and disposition. A failed control blocks result interpretation until the cause is resolved.
Interpretation and confirmation pathway
Typical colonies of Burkholderia cepacia are circular, vary from grey to sage green, with the medium changing from orange to brightpink.
Record the raw observation before the conclusion: morphology or colour, growth pattern, endpoint, reaction intensity, reading time, and control response. Presumptive reactions remain presumptive. Route atypical, weak, mixed, or delayed reactions through the confirmation step in the current method.
Storage, stability, and pre-use inspection
AND TRANSPORT CONDITIONS6-12°C away from light, until the expiry date on the label. However, our stability studies have shown that the transport at 18-25°C for 4days, or at 35-39°C for 48 hours, does not alter in any way the
Receipt and storage records should reconstruct temperature exposure, lot rotation, expiry, container integrity, and time outside controlled storage. Inspect colour, clarity, fill volume, gel integrity, contamination, dehydration, and precipitate before use.
Limitations and boundaries of use
The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed forIn vitro diagnostic use and must be used only by properly trained operators.
Applicability depends on matrix, target organism, interfering flora, inoculum, validated method, and regulatory setting. Do not transfer conditions or acceptance criteria from a different matrix without verification.
Troubleshooting from evidence, not guesswork
- Poor recovery: check preparation, pH, overheating, supplement addition, inoculum, atmosphere, storage, and control performance.
- Loss of selectivity: review formulation, sterilization, expiry, inoculum burden, enrichment sequence, and incubation time.
- Weak or atypical reaction: confirm reading time and atmosphere, compare controls, document mixed growth, and follow confirmation requirements.
- Run-to-run drift: trend lot, analyst, equipment, water, preparation batch, controls, and environmental conditions.
External method context
The product should be understood as one measurement component in an antimicrobial susceptibility method. The analytical chain includes isolate purity, standardized inoculum, medium composition, antimicrobial concentration, incubation atmosphere and time, endpoint reading, and the interpretive table selected by the laboratory. A shift in any upstream variable can move an MIC or zone without a change in organism biology, which is why QC distributions and trend review are more informative than a single pass/fail control.
External laboratory-quality guidance also supports a lifecycle view: verify the method when introduced, bridge new lots against an accepted lot, trend control results, investigate nonconformities, and retain enough raw observations to reconstruct the decision. This is the practical difference between reproducing a preparation instruction and demonstrating that the resulting measurement is fit for its intended use.
EUCAST disk diffusion and quality control
EUCAST requires standardized methodology, current breakpoint tables, and routine quality control; the laboratory remains responsible for the final susceptibility report. For broth dilution, inoculum density, cation content, pH, incubation atmosphere, endpoint definition, and two-fold dilution design influence MIC reproducibility. Breakpoints are interpretive rules applied after a technically valid MIC or zone measurement; they are not properties of the product alone. Review the authoritative source.
FDA Bacteriological Analytical Manual
FDA BAM methods organize food microbiology around validated sampling, preparation, enrichment, isolation, and confirmation steps, with method applicability tied to the matrix and target organism. Sampling uncertainty, homogenization efficiency, inhibitory ingredients, dilution design, and countable range can dominate the result before the culture medium is ever inoculated; those variables belong in method verification and uncertainty review. Review the authoritative source.
WHO Laboratory Quality Management System handbook
WHO laboratory quality guidance treats document control, equipment, purchasing and inventory, process control, records, occurrence management, and continual improvement as connected parts of reliable testing. Review the authoritative source.
Technical references
- Manufacturer product documentation for catalogue 11506.
- EUCAST disk diffusion and quality control (access checked 2026-07-20).
- FDA Bacteriological Analytical Manual (access checked 2026-07-20).
- WHO Laboratory Quality Management System handbook (access checked 2026-07-20).
Bench-use checklist
- Verify product identity, catalogue number, lot, expiry, and storage history.
- Document preparation and incubation exactly as controlled in the SOP.
- Define controls and acceptance criteria before testing.
- Record raw observations, control response, reading time, and confirmation decision.
- Escalate failed controls, atypical reactions, and departures through the quality system.