Product and use case
Liofilchem Tryptone Soya Yeast Extract Broth Culture Media 620241 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is tryptone Soya Yeast Extract Broth (TSYEB) is a liquid medium used for the isolation and cultivation of Listeria spp in foods.This medium complies with the recommendations given in ISO 11290, APHA and FDA for the detection and enumeration of Listeria monocytogenes.
This product supports Listeria workflows in ready-to-eat food, dairy, meat, and environmental monitoring programs where early presumptive recovery must lead into confirmation.
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When this format is useful
Use this product where the lab must protect a recovery step before confirmation. For ready-to-eat food and environmental samples, the important decision is not only whether growth appears, but whether the enrichment or plating step gives the organism enough opportunity to recover from stress, competing flora, preservatives, or cold-chain exposure.
What to read after incubation
OF RESULTS Refer to appropriate
Selective or enrichment logic
Tryptone and soya peptone provide nitrogen, vitamins, minerals and amino acids for organisms growth. Yeast extract is a source of vitamins, particularly of the B-group. Glucose is the fermentable carbohydrate providing carbon and energy. Dipotassium phosphate is the buffer. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
Sample handling workflow
- Preparation: Suspend 36 g of powder in 1 liter of distilled water. Mix well and dissolve by heating with frequent agitation. Dispense into appropriate containers. Autoclave at 121°C for 15 minutes.
- Procedure: Take an isolated colony obtained in TSYEA (ref. 610367) and suspend in a tube of TSYEB. Incubate at 25°C for 8-24 h until a cloudy medium is observed. Use a drop of this broth culture to examine for motility at the microscope. A culture from TSYEB can be used for inoculating carbohydrate utilization broths and other test media. This culture may be kept at 4°C several days and used repeatedly as inoculum.
Technical verification points
- Formula / composition: (g/l)Tryptone17.0Soya Peptone 3.0Yeast Extract 6.0Sodium Chloride 5.0Dipotassium Phosphate 2.5D-Glucose 2.5Final pH 7.3 ± 0.2 at 25°C
Safety and documentation points
- Hazards: 2.1 Clas sification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 La bel elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74620241
- Catalog/document code matched: 620241, 11290
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/tryptone-soy-yeast-extract-b-unit-100-g