Application snapshot
Liofilchem CARY Blair Transport Medium Culture Media 611402 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is cary Blair Transport Medium is used for transportation and preservation of clinical specimens, primarily faecal and rectal samples. This medium has demonstrated its effectiveness in recovery of various microorganisms like Vibrio parahaemolyticus and species of Salmonella, Shigella and Yersinia.
This product is relevant to food safety and environmental monitoring programs where Salmonella screening is part of raw material, finished product, or hygiene verification testing.
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Application fit for the lab
This product fits screening programs where a negative or presumptive result can affect raw-material release, finished-product hold decisions, or hygiene investigations. The lab should connect the medium to its enrichment sequence, confirmation method, and written rule for when suspect colonies move forward.
Expected colonies or reaction pattern
This medium serves as a vehicle for maintaining the viability of enteric bacterial pathogens during transport and
Principle behind the medium
The low nutrient content is effective in maintaining the viability of microorganisms without significant increase in growth. Sodium thioglycollate is a reducing agent. Disodium phosphate acts as buffer. Calcium chloride and sodium Chloride provide essential ions that help maintain the osmotic balance of the medium while controlling permeability of bacterial cells. Agar is the solidifying agent. The high pH is an aid for recovery of Vibrio spp.
How to build it into the SOP
- Preparation: Notes: 1.Sufficient volume of medium must be dispensed into containers so that swabs will be covered completely. 2.Store ready-to-use tubes at 2-8°C, or other validated temperature, until used. It is responsibility of the individual laboratory to establish
- Procedure: 1.Allow tubes to come to room temperature. 2.a) Swab specimen: Immerse swab into the medium and cut off swab shaft. b) Stool specimen: Place approximately one gram of the faecal specimen into the medium and agitate to permit adequate mixing of the specimen with the transport medium. 3.Replace cap, tighten and label with appropriate patient information. 4.Transport the specimen to the laboratory for processing with minimal delay. Follow established laboratory procedures during transport and prior to processing (e.g. specimen should be maintained at 2-8°C during shipment if recovery of certain enteric pathogens such as Shigella and Campylobacter is required). 5.Mix the specimen thoroughly prior to inoculation of appropriate media.
Composition and QC checkpoints
- Formula / composition: * (g/litre) *Adjusted and/or supplemented as required to meet
Handling limits and documentation
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for in vitro diagnostic use only and must be used by properly trained operators.
- Storage / shelf life: temperature and expiration date of the medium in its final containers.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Skin Sens. 1; Skin sensitization (Category 1), H317 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H317 May cause an allergic skin reaction. Precautionary statement(s) P261 P280 P302 + P352 P333 + P313 P363 Avoid breathing dust. Wear protective gloves/protective clothing.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74611402
- Catalog/document code matched: 611402, 2020
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/cary-blair-transport-medium-for-microbiology-lab-testing