Product overview
Liofilchem Azide Maltose Agar KF Culture Media 610154 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is aZIDE MALTOSE AGAR KF is a selective medium utilized for isolating and enumerating the fecal streptococci in waters and foods. APHA, FDA, ICMSF recommend the medium for the primary isolation of fecal streptococci in waters and foodstuffs, by the
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Where this product fits
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Result signals and confirmation
free-flowing, homogeneous. Color: light-greenish beige. Prepared medium
Bench workflow
- Procedure: of membrane filtration or by poured plate method. Sodium azide inhibits growth of Gram-negative bacteria, of streptococci not belonging to group D and of lactic bacteria (Leuconostoc mesenteroides, Lactobacillus lactis, Lactobacillus acidophilus). Fecal streptococci reduce T.T.C. and appear with pink-red colonies.
Controls and performance checks
- Formula / composition: (g/l) Peptospecial 10.0 Yeast Extract 10.0 Sodium Chloride 5.0 Sodium Glycerophosphate 10.0 Maltose 20.0 Lactose 1.0 Sodium Azide 0.4 Brom Cresol Purple 0.015 Agar 20.0 Final pH = 7.2 ± 0.2 at 25 °C. DIRECTIONS Suspend 76.4 g of powder in 990 ml of distilled or deionized water. Heat to boiling for approximately 5 minutes and gently agitate until completely dissolved. DO NOT STERILIZE. Cool to 45-50 °C and aseptically add 10 ml of T.T.C. 1% supplement (code 80300). Mix well. Dispense in petri dishes.
Storage, limits, and safety
- Limitations: Many strains of S. bovis and S. equinus are inhibited by azide. Overheating may lower pH, causing the decrease of productivity by medium.
- Warnings / precautions: Azide Maltose Agar KF contains sodium azide. This substance is harmful by inhalation and if swallowed, irritating to eyes, respiratory system and skin. Consult safety data sheet for further details. Sodium azide reacts with many metals, especially copper, to produce explosive metal azides.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared media at 2-8 °C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Acute Tox. 4, Acute toxicity, Oral (Category 4); H302 Aquatic Chronic 3, Chronic aquatic toxicity (Category 3); H412 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H302 H412 Harmful if swallowed. Harmful to aquatic life with long lasting effects.
- Exposure controls: 8.1 Control parameters • Sodium azide (CAS 26628-22-8) Indicative occupational exposure limit values (IOELV) Short term: 0,3 mg/m3 Long term: 0,1 mg/m3 SAFETY DATA SHEET (According to Regulation EC n° 1907/2006 and to Commission Regulation EU 2020/878) Revision: 4 / Revision Date: 20.11.2023 ©Liofilchem s.r.l. Via Scozia 64026, Roseto degli Abruzzi (TE) Italy - Tel +39 0858930745 - Fax +39 0858930330 MSDS Ref.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product m ust be sp ecially treated adhering to official regulations administrative. Offer surplus and non - recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610154
- Catalog/document code matched: 610154
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/azide-maltose-agar-kf-unit-500-g