Laboratory use profile
Liofilchem Columbia Agar Base Culture Media 610013 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is cOLUMBIA AGAR BASE, enriched with sterile sheep blood (5%), is suitable for isolation and growth of fastidious microorganisms such as streptococci, staphylococci, pneumococci and listeriae from clinical samples.
This product supports Listeria workflows in ready-to-eat food, dairy, meat, and environmental monitoring programs where early presumptive recovery must lead into confirmation.
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Use case and sample types
Use this product where the lab must protect a recovery step before confirmation. For ready-to-eat food and environmental samples, the important decision is not only whether growth appears, but whether the enrichment or plating step gives the organism enough opportunity to recover from stress, competing flora, preservatives, or cold-chain exposure.
Decision criteria after incubation
free-flowing, homogeneous. Color: beige. Prepared medium
Preparation and execution notes
- Procedure: Inoculate the medium with the specimen streaking by a sterile loop and incubate at 36 ± 1 °C for 18-48 hours aerobically, anaerobically or under conditions of increased CO2 (5-10%), in accordance with established laboratory procedures. Examine plates for growth and hemolytic reactions. Four types of hemolysis on blood agar media can be described: 1. α-hemolysis is the reduction of hemoglobin to methemoglobin in the medium surrounding the colony, causing a greenish discolorization of the medium. 2. beta-hemolysis is the lysis of red blood cells, producing a clear zone surrounding the colony. 3. γ-hemolysis indicates no destruction of red blood cells and no change in the color of the medium. 4. ά-hemolysis indicates a partial lysis.
Formula, QC, and performance
- Formula / composition: (g/l) Peptospecial 23.0 Starch 1.0 Sodium Chloride 5.0 Agar 14.0 Final pH = 7.3 ± 0.2 at 25 °C. DIRECTIONS Suspend 43.0 g of powder in 1 liter of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C and aseptically add 5% defibrinated sterile sheep blood. Mix well. Dispense in petri dishes.
Risk controls for routine use
- Limitations: When this medium is enriched with 10% sterile sheep blood, heated at 80 °C for 10 minutes until a chocolate color is obtained, and an antibiotic mixture is added (vancomycin, colimycin, trimethoprim, amphoterycin B) it is suitable for the selective isolation of the pathogens neisseria. If used without the addition of blood, the medium is suitable for growing of Brucella abortus, Yersinia pestis, Clostridium perfrigens and enterobacteria. Hemolytic reactions of some strains of Group D streptococci have been shown to be affected by differences in animal blood.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8 °C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610013
- Catalog/document code matched: 610013
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/columbia-agar-base-for-microbiology-lab-testing