Laboratory use profile
Liofilchem TRIBUTYRIN Agar Base Culture Media 610215 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is tRIBUTYRIN AGAR BASE is a basal medium for detection and enumeration of lipolytic microorganisms in foodstuffs and othermaterials. The medium can also be used for the detection of lipase in various bacterial species such as staphylococci, clostridia,Pseudomonas, marine flavobacteria etc.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Use case and sample types
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Decision criteria after incubation
Lipolytic microorganisms produce colonies which are surrounded by clear zones in the otherwise turbid culture medium.
Method logic
Peptone and yeast extract provide nitrogen and vitamins required for organism growth. Agar is the solidifying agent. The culture mediummust be supplemented with tributyrin (GLYCERYL TRIBUTYRATE, ref. 80301). This compound gives rise to clear zones surrounding thelipolytic colonies in the otherwise turbid culture medium.
Preparation and execution notes
- Preparation: Suspend 23 g of powder in 1000 ml of distilled water. Heat until completely dissolved. Add 10 ml of GLYCERYL TRIBUTYRATE(ref. 80301). Mix thoroughly. Autoclave at 121°C for 15 minutes. While shaking frequently (emulsification of the tributyrin) cool to45-50°C. Pour in petri dishes.
- Procedure: Inoculate the culture medium by spreading the the sample material on the surface of the plates. Incubate at 28°C up to 72 hours.
Formula, QC, and performance
- Formula / composition: (g/l)Peptone5.0Yeast Extract3.0Agar15.0Final pH 7.5 ± 0.2 at 25°C
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: 10-100 CFU/mlInoculum for selectivity: 104-105 CFU/mlInoculum for specificity: ≤104 CFU/mlIncubation conditions:18-24 h at 36 ± 1°CMicroorganismATCCClear zones surrounding the colonies Escherichia coli25922-Salmonella typhimurium14028-Pseudomonas aeruginosa27853+Staphylococcus aureus25923+Bacillus subtilis6633+TABLE OF SYMBOLS Batchcode Keep away fromheat sources Manufacturer Use by Fragile, handlewith care Cataloguenumber Temperaturelimitation Contains sufficientfor <n> tests Consult
Risk controls for routine use
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product must be usedonly by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use itbefore the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8°C away fromlight.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to national and local regulations in force.REFERENCES1.Anderson J.A. (1939) The use of tributyrin agar in dairy bacteriology. Ber. 3. Int. Mikrobiol. Kongress, 3:726-278.2.Innes A.G. (1956) Coagulase positive staphylococci from bulk milk supplies low in solids-notfat. J. Appl. Bact., 19:39-45.3.Hayes P.R. (1963) Studies on marine flavobacteria. J. Gen. Microbiol., 30:1-19.4.Willis A.T. (1960) The lipolytic activity of some clostridia. J. Path. Bact., 80:379-390.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610215
- Catalog/document code matched: 610215
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/tributyrin-agar-base-for-microbiology-lab-testing