Laboratory use profile
Liofilchem Listeria OXFORD Agar Culture Media 610167 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is lISTERIA OXFORD AGAR, is a selective medium for the isolation of Listeria spp in foods, milk and dairy products, environmental and clinical specimens after enrichment in the appropriate broth, according to ISO 10560: 1993. The selectivity is due to cycloeximide which inhibits the growth of fungi and to phosphomycin and cefotetan which inhibit containing bacteria. The presence of aesculin and ferric ammonium citrate allows a presumptive identification of the black colonies. In fact the Listeria species hydrolize the aesculin to glucose and esculetin which reacts with the ferric ions in the medium. Listeria spp colonies can appear in dark/black color surrounded by a zone of the same color.
This product supports Listeria workflows in ready-to-eat food, dairy, meat, and environmental monitoring programs where early presumptive recovery must lead into confirmation.
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Use case and sample types
Use this product where the lab must protect a recovery step before confirmation. For ready-to-eat food and environmental samples, the important decision is not only whether growth appears, but whether the enrichment or plating step gives the organism enough opportunity to recover from stress, competing flora, preservatives, or cold-chain exposure.
Decision criteria after incubation
free-flowing, homogeneous. Color: beige. Prepared medium
Preparation and execution notes
- Procedure: Streak a loopful of the suitable enriched broth, incubated with the sample to analyze, onto the surface of the medium. Incubate at 36 ± 1°C for 24-48 hours. Observe for the presence of Listeria typical colonies that are grey-brown with brown or black halo.
Formula, QC, and performance
- Formula / composition: (g/l) Peptospecial 10.0 Peptone 3.0 Tryptose 10.0 Maize Starch 1.0 Sodium Chloride 5.0 Esculin 1.0 Ferric Ammonium Citrate 0.5 Lithium Chloride 15.0 Colistin Sulphate 0.02 Cycloeximide (Actidione) 0.4 Acriflavine 0.005 Agar 15.0 Final pH = 7.0 ± 0.2 at 25 °C. DIRECTIONS Suspend 60.9 g of powder in 1 liter of distilled or deionized water. Heat until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50°C.
Risk controls for routine use
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8 °C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Acute Tox. 3, Acute Toxicity, Orale (Category 2); H301 Skin Irrit. 2, Skin Irritation (Category 2); H315 Eye Irrit. 2, Eye Irritation (Category 2); H319 Repr.
- Exposure controls: 8.1 Control parameters • Lithium chloride (CAS 7447-41-8) DNEL (Derived No Effect Level): worker, acute, by inhalation: 30 mg/m3 worker, long term, by inhalation: 10 mg/m3 user, long term, by inhalation: 10 mg/m3 • PNEC (Predicted No-Effect Concentration): fresh water: 10.4 mg/l freshwater sediment: 49.9 mg/kg sea water 1.004 mg/l marine sediment: 4.99 mg/kg soil: 4.13 mg/kg sewage treatment plant: 140.2 mg/L 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be sp ecially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610167
- Catalog/document code matched: 610167, 10560
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/listeria-oxford-agar-for-microbiology-lab-testing