Application snapshot
Liofilchem PHENOL Red Agar Base Culture Media 610308 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is pHENOL RED AGAR with added carbohydrate is well suited for the study of fermentation reactions of microorganisms.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Application fit for the lab
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Expected colonies or reaction pattern
Fermentation of the carbohydrate is indicated by a change in the color of the medium from red to canary yellow. Gas formation is indicated by the collection of gas bubbles in the base or by splitting of the agar. For expected reactions with organisms on PHENOL RED AGAR supplemented with various carbohydrates, refer to appropriate
Principle behind the medium
Peptone provides the carbon and nitrogen required for good growth of a wide variety of organisms. Sodium chloride maintains the osmotic balance of the medium. Agar is the solidifying agent. Phenol red serves as a pH indicator, turning from red-orange to yellow when acid is produced during fermentation of the carbohydrate; if the carbohydrate is not fermented, the medium remains red or becomes alkaline (darker red).
How to build it into the SOP
- Preparation: 1. Suspend 30 g of the powder in 1 L of purified water. Heat with frequent agitation to completely dissolve the powder. 2. Autoclave at 121°C for 15 minutes. Aseptically add carbohydrate, 5-10 g per L, if desired. Mix thoroughly. 3. Dispense into final tubes and allow to solidify in a slant position.
- Procedure: 1. Inoculate the medium by stabbing into the butt and streaking the surface of the slant. If desired, inoculate obligate anaerobic bacteria into melted medium that has been cooled to 45°C. Allow the agar to solidify prior to incubation. 2. Incubate at 36 ± 1°C for 4-48 hours (or anaerobically for 24-72 hours). 3. Examine periodically for growth, acid production and gas formation.
Composition and QC checkpoints
- Formula / composition: (g/L) Tryptone 10.0 Sodium Chloride 5.0 Phenol Red 18.0 mg Agar 15.0 Final pH = 7.4 ± 0.2 at 25°C
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74610308
- Catalog/document code matched: 610308
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/phenol-red-agar-base-for-microbiology-lab-testing