Product overview
Liofilchem VOGEL JOHNSON Agar Culture Media 610186 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is vOGEL JOHNSON AGAR, by selecting and identifying coagulase positive and mannitol-fermenting strains, permits the early detection of Staphylococcus aureus from heavily contaminated foods and clinical specimens. It corresponds to the specification of the United States Pharmacopoeia in terms of its
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
View this product on Kormay Biomedicals
Where this product fits
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Result signals and confirmation
free-flowing, homogeneous. Color: pink. Prepared medium
Bench workflow
- Procedure: With a glass spatula spread from 0.1 to 1.0 ml of the specimen or of diluted food (macerated in 0.1% Peptone Water) onto the surface of a well dried plate. Incubate at 36 ± 1 °C for 24-48 hours. Staphylococcus aureus is able to reduce tellurite to metallic tellurium resulting in growth as black colonies surrounded by a yellow zone.
Controls and performance checks
- Formula / composition: (g/l) Tryptone 10.0 Yeast Extract 5.0 Mannitol 10.0 Dipotassium Phosphate 5.0 Lithium Chloride 5.0 Glycine 10.0 Phenol Red 0.025 Agar 15.0 Final pH = 7.2 ± 0.2 at 25 °C. DIRECTIONS Suspend 60.0 g of powder in 1 liter of distilled or deionized water. Heat until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50°C and aseptically add 20 ml of Potassium Tellurite 1% supplement (code 80022). Final medium will contain 0.01 mg of potassium tellurite per litre. Dispense in petri dishes.
Storage, limits, and safety
- Limitations: Prolonged incubation may result in the growth of black coagulase-negative colonies and if these organisms also ferment mannitol, they may be falsely identified from their
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8°C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610186
- Catalog/document code matched: 610186
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/vogel-johnson-agar-for-microbiology-lab-testing