Laboratory use profile
Liofilchem FERMENTATION Broth Base Culture Media 610030 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is fermentation Broth Base is a liquid medium used with added carbohydrate in differentiating pure cultures of bacteria. This medium is formulated according to ISO 11290 and is recommended for the confirmation test of Listeria monocytogenes.
This product supports Listeria workflows in ready-to-eat food, dairy, meat, and environmental monitoring programs where early presumptive recovery must lead into confirmation.
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Use case and sample types
Use this product where the lab must protect a recovery step before confirmation. For ready-to-eat food and environmental samples, the important decision is not only whether growth appears, but whether the enrichment or plating step gives the organism enough opportunity to recover from stress, competing flora, preservatives, or cold-chain exposure.
Decision criteria after incubation
Observe daily for colour change from purple to yellow (acid production) and gas formation.
Method logic
Peptone and meat extract supply amino acids, nitrogen, carbon, minerals, vitamins and other nutrients which support the growth of microorganism. Sodium chloride maintains the osmotic balance of the medium. Bromocresol purple is the pH indicator.
Preparation and execution notes
- Preparation: Suspend 16.0 g of powder in 1 liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Dispense into tubes containing inverted Durham tubes. Sterilize at 121°C for 15 minutes. Cool up to 45-50°C. Add the suitable carbohydrate solution to give a final concentration of 0.5%. Mix well.
- Procedure: Inoculate each tube with a loopful of a pure culture. Incubate at 37°C for up to 7 days.
Formula, QC, and performance
- Formula / composition: (g/l)Proteose Peptone10.0Meat Extract 1.0Sodium Chloride 5.0Bromocresol Purple 0.02Final pH 6.8 ± 0.2 at 25°C
Risk controls for routine use
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for professional use only and must be used by properly trained operators.
- Storage / shelf life: AND TRANSPORT CONDITIONSThe powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610030
- Catalog/document code matched: 610030, 11290
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/fermentation-broth-base-for-microbiology-lab-testing