Product and use case
Liofilchem Yeast Extract Agar Culture Media 611016 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is yeast Extract Agar is a nutrient medium used for the determination of total microbial count in all types of water in accordance with the recommendations of ISO 6222.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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When this format is useful
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
What to read after incubation
Count colonies on each plate (reject any plate with confluent growth) and express the results as CFU/ml of sample allowing for dilution factors.
Selective or enrichment logic
Enzymatic digest of casein provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Yeast extract is a source of vitamins, particularly of B-group. Agar is the solidifying agent.
Sample handling workflow
- Preparation: Dehydrated medium Suspend 24 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Medium in tubes/bottlesMelt the content of the tube/bottle in a water bath at 100°C (loosing the cap partially removed) until completely dissolved. Then screw the cap and check the homogeneity of the dissolved medium, if it is the case turning the tube/bottle upside down. Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into Petri dishes.
- Procedure: 1.Make dilutions of the test sample taking into account the level of pollution expected. 2.Inoculate the medium (two sets of plates for each sample) by pour plating or membrane filtration method. 3.Incubate one set of plates at 36 ± 2°C for 40-48 h and the other set at 22 ± 2°C for 64-72 h.
Technical verification points
- Formula / composition: (g/l) Enzymatic Digest of Casein 6.0 Yeast Extract 3.0 Agar 15.0 Final pH 7.2 ± 0.2 at 25°C
- Quality control: Plates are inoculated with the microbial strains indicated in the QC table. Inoculum for productivity: 50-100 CFU Incubation conditions: aerobically at 36 ± 2°C for 40-48 hours. QC Table. Microorganism Growth Escherichia coli WDCM 00012 Good Bacillus subtilis WDCM 00003 Good WARNING AND
Storage, hazards, and disposal
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended professional use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store bottles, tubes and prepared plates at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards Results of PBT and vPvB assessment • PBT: Not applicable. • vPvB: Not applicable 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product mu st be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74611016
- Catalog/document code matched: 611016, 2015, 6222
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/yeast-extract-agar-units