Application snapshot
Liofilchem Muller KAUFFMANN TETRATHIONATE Broth Base Culture Media 620239 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is muller Kauffmann Tetrathionate Broth Base is used with supplements for the selective enrichment of Salmonella in food, animal feed and environmental samples. The complete medium complies with ISO 6579-1 requirements.
This product is relevant to food safety and environmental monitoring programs where Salmonella screening is part of raw material, finished product, or hygiene verification testing.
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Application fit for the lab
This product fits screening programs where a negative or presumptive result can affect raw-material release, finished-product hold decisions, or hygiene investigations. The lab should connect the medium to its enrichment sequence, confirmation method, and written rule for when suspect colonies move forward.
Expected colonies or reaction pattern
Turbidity indicates microbial growth.Presumptive identification is achieved by subculture onto XLD Agar (ref. 10056) and a second selective agar of choice such as Chromatic Salmonella (ref. 11614). Characteristic presumptive Salmonella colonies should be confirmed with biochemical and serological tests.
Principle behind the medium
Enzymatic digest of casein and meat extract provide amino acids, nitrogen, carbon, vitamins and minerals. Sodium chloride maintains the osmotic balance of the medium. Calcium carbonate is the buffer. Sodium thiosulfate is included to produce tetrathionate after addingiodine to the medium. Organisms-reducing tetrathionate, such as Salmonella, grow luxuriant while most faecal organisms are inhibited. Bile promotes the growth of Salmonella while inhibiting the contaminant bacterial flora. Brilliant green suppresses primarily Gram-positive bacteria. Novobiocin is added to inhibit Gram-positive bacteria.
How to build it into the SOP
- Preparation: Suspend 89.5 g of powder in 1 liter of deionized or distilled water. Heat with frequent agitation and boil for 5 minutes to completely dissolved the powder. DO NOT AUTOCLAVE. Cool up to 45-50°C. Aseptically, add the contents of 2 tubes (20 ml) of Iodine MKTT Solution (ref. 80009). Also add the contents of 2 vials of Novobiocin MKTT Supplement (ref. 81073) each reconstituted with 5 ml sterile distilled water. Mix well. Dispense into sterile containers.
- Procedure: For pre-enrichment, add the sample to Buffered Peptone Water (ref. 414020) at a ratio of 1:9 (e.g. 25 g per 225 ml), homogenize well and incubate at 36 ± 2°C for 16-20 h.Transfer 1 ml of the pre-enrichment culture to a tube containing 10 ml of Muller Kauffmann tetrathionate-novobiocin (MKTTn) broth. Incubate at 37 ± 1°C for 18-24 h.
Composition and QC checkpoints
- Formula / composition: (g/l)Enzymatic Digest of Casein 8.6Meat Extract 4.3Sodium Chloride 2.6Calcium Carbonate38.7Sodium Thiosulfate anhydrous30.5*Ox Bile 4.78Brilliant Green 0.0096Final pH 8.0 ± 0.2 at 25°C*Equivalent to 47.8 g of sodium thiosulfate pentahydrate.
Handling limits and documentation
- Warnings / precautions: For professional use only. Operators must be trained and have certain experience in the laboratory methods. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from theinstructions in this document.Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.REFERENCES1.ISO 6579-1:2017+Amd1:2020. Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 1: Detection of Salmonella spp. — Amendment 1: Broader range of incubation temperatures, amendment to the status of Annex D, and correction of the composition of MSRV and SC2.DeSmedit J.M., R. Bolderdijk, H. Rappold and D.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The produ ct must be sp ecially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74620239
- Catalog/document code matched: 620239, 6579
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/muller-kauffmann-tetrathionate-broth-base-for-microbiology-lab-testing