Product overview
Liofilchem Chromatic CRE Agar Base Culture Media 611619 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is chromaticTM CRE Agar Base is a chromogenic selective medium used with supplements for detection of carbapenem-resistant Enterobacteriaceae directly from clinical specimens.Carbapenems (imipenem, meropenem, ertapenem and doripenem) are the last shelter from multi-resistant Gram-negative bacterial infections. Carbapenemase is an enzyme class which can be produced by Klebsiella pneumoniae and by other organisms including Serratia spp, Enterobacter spp, Escherichia coli and Citrobacter freundii. Early detection of carbapenems resistance is essential in orderto limit the spread of these pathogens.
This product is relevant to selective chromogenic screening and presumptive differentiation. It is best positioned for clinical, food safety, infection-control, and industrial microbiology labs that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Where this product fits
Use this product where indicator organisms are part of a release, trend, or sanitation decision. The practical value comes from separating countable or presumptive target reactions from background flora so the analyst can document whether the sample meets the site limit.
Result signals and confirmation
K. pneumoniae produces blue-violet colonies. E. coli produces red colonies.Enterobacter spp, produces blue-green colonies. Citrobacter spp, produces blue colonies with red halo.Non-Enterobacteriaceae (if not inhibited) produce white to naturally pigmented colonies.
Method principle
Peptone mix is a source of amino acids, nitrogen, minerals, vitamins, and other factors which increases the growth of bacteria. Chromogenic and selective mix facilitates the identification of bacteria on the basis of the color and colony morphology. Agar is the solidifying agent.Supplementation with ChromaticTM CRE Supplement (ref. 81088) inhibits most of the non-Enterobacteriaceae and the carbapenem-sensitive organisms.
Bench workflow
- Preparation: Suspend 56.8 g of powder in one liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Sterilize at 121°C for 15 minutes. Cool up to 45-50°C. Aseptically, add the contents of 2 vials (10 ml) of ChromaticTM CRE Supplement (ref. 81088) reconstituted as directed in the instructions for use that accompany the product. Pour in Petri dishes.
- Procedure: Inoculate the medium directly from faecal screening swabs, or using a sterile loop previously dipped in liquid suspension of the sample, by streaking the specimen onto the agar surface. Incubate aerobically at 37°C for 18-24h.
Controls and performance checks
- Formula / composition: (g/l)Peptone Mix40.0Chromogenic and Selective Mix 1.8Agar15.0Final pH 7.0 ± 0.2
Storage, limits, and safety
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for In vitro diagnostic use and must be used only by properly trained operators.
- Storage / shelf life: CONDITIONS The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74611619
- Catalog/document code matched: 611619
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/chromatic-cre-agar-base-for-microbiology-lab-testing