Laboratory use profile
Liofilchem SS Agar (MODIFIED) Culture Media 610042 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is highly selective medium for the isolation of Salmonella spp. and some species of Shigella from clinical specimens, food and other types of samples. This medium is intended as an aid in the diagnosis, requiring further tests to complete the diagnostic results.
This product is relevant to food safety and environmental monitoring programs where Salmonella screening is part of raw material, finished product, or hygiene verification testing.
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Use case and sample types
This product fits screening programs where a negative or presumptive result can affect raw-material release, finished-product hold decisions, or hygiene investigations. The lab should connect the medium to its enrichment sequence, confirmation method, and written rule for when suspect colonies move forward.
Decision criteria after incubation
Salmonella spp. produce transparent colonies with a black center. Shigella spp. form light rose to colorless colonies. Non-lactose fermenters (supposed pathogens) produce colorless colonies. Some coliforms, which might not be inhibited, will form small colonies from pink to red in color.
Method logic
Peptone and meat extract provide amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Lactose is the fermentable carbohydrate. Bile salts, sodium citrate and brilliant green inhibit gram-positive bacteria and several Enterobacteriaceae, while allowing species of Salmonella and Shigella to grow. Sodium thiosulfate and ferric ammonium citrate enable the detection of hydrogen sulfide production as evidenced by colonies with black center. Neutral red is the pH indicator. Agar is the solidifying agent.
Preparation and execution notes
- Preparation: Dehydrated medium. Suspend 52.0 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until dissolved completely. DO NOT AUTOCLAVE. Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into Petri dishes.
- Materials required: Standard microbiological supplies and equipment such as: Sterile Petri plates, test tubes, inoculating loops, swabs, incubator,
- Procedure: Inoculate the medium heavily with the specimen as soon as possible after it is received in the laboratory. The streak plate
Formula, QC, and performance
- Formula / composition: * (g/litre) Peptone 5.5 Meat Extract 5.0 Lactose 10.0 Sodium Thiosulphate 8.5 Yeast Extract 5.0 Sodium Citrate 1.0 Bile Salts No.3 1.5 Ferric Ammonium Citrate 1.5 Brilliant Green 0.00033 Neutral Red 0.025 Agar 14.0 Final pH 7.0 ± 0.2 at 25°C *Adjusted and/or supplemented as required to meet
- Quality control: organisms. SPECIMENS Clinical specimens such as stool specimens or rectal swabs of patients suspected to have a bacterial enteric infection should be sampled at the acute stage, before antimicrobial therapy (where possible) and examined as soon as possible after collection. Good laboratory practices for collection, transport and
Risk controls for routine use
- Limitations: Invalid results can be caused by poor specimen quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the principles of the procedures, including its
- Storage / shelf life: of the clinical specimens should be applied. Refer to specific guidelines for more information about specimen collection and
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610042
- Catalog/document code matched: 610042, 2023, 00033
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/ss-agar-modified-for-microbiology-lab-testing