Product and use case
Liofilchem STAPHYLOCOCCUS 110 Agar Culture Media 611366 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is sTAPHYLOCOCCUS 110 AGAR is a medium for isolation and differentiation of staphylococci based on mannitol fermentation, pigment formation and gelatinase activity.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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When this format is useful
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
What to read after incubation
free-flowing, homogeneous. Color: very light beige to beige. Prepared medium
Sample handling workflow
- Procedure: Blend 20 g of specimen with sterile 80 ml 0.2 M NaCl solution for 3 minutes at high speed. Prepare tenfold dilutions and place 0.1 ml of each dilution onto prepared Staphylococcus 110 Agar plates. Spread with a sterile bent glass rod the material to examine on the surface of the solidified medium. Incubate at 36 ± 1°C for 18-48 hours. Growth of pathogenic staphylococci produces colonies of yellow-orange pigment. To test for gelatinase reaction, flood the plate with 5 ml of ammonium sulphate saturated solution and incubate at 36 ± 1 °C for 10 minutes. Observe for a zone of clearing around the colonies (positive reaction).
Technical verification points
- Formula / composition: (g/l) Tryptone 10.0 Yeast Extract 2.5 Lactose 2.0 Gelatin 30.0 D-Mannitol 10.0 Sodium Chloride 75.0 Dipotassium Phosphate 5.0 Agar 15.0 Final pH = 7.0 ± 0.2 at 25 °C. DIRECTIONS Suspend 149.5 g of powder in 1 liter of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C. Dispense in petri dishes.
Storage, hazards, and disposal
- Limitations: Enterococcus faecalis may grow on Staphylococcus 110 Agar as tiny colonies with mannitol fermentation. Differentiate these organisms from staphylococci with the Gram stain and catalase test. Suspected Staphilococci must be subcultured to Nutrient Broth or Blood Agar for coagulase test as the high salt content of Staphilococcus 110 Agar may interfere with results. Pigment production is not reliable criterion for differentiation of staphylococcal spp.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8 °C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74611366
- Catalog/document code matched: 611366
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/staphylococcus-110-agar-for-microbiology-lab-testing