Laboratory use profile
Liofilchem ANAEROBIC Agar (BREWER) Culture Media 610320 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is anaerobic Agar (Brewer) is a nonselective culture medium used for the cultivation of facultative and strict anaerobes, especially Clostridium species. This medium is based on a
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Use case and sample types
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Decision criteria after incubation
Observe the microbial growth.
Method logic
Peptones provide nitrogen, carbon, amino acids, vitamins and minerals for organism growth. Yeast extract is a source of vitamins, particularly of group B. Glucose is the fermentable carbohydrate. Sodium chloride maintains the osmotic balance of the medium. Sodium thioglycollate and sodium formaldehyde sulfoxylate are the reducing agents. Resazurin is an indicator of anaerobiosis. Agar is the solidifying agent.
Preparation and execution notes
- Procedure: Tests can be performed using Standard Petri Dishes or Brewer Anaerobic Agar Plates. Inoculate the medium either by streaking the sample onto the agar surface or by the pour plate method. Immediately incubate anaerobically at 35 ± 2°C for 18-48 hours. Longer incubation time may be necessary to recover some anaerobes. Notes: 1.If the medium has not been prepared fresh before use, it is necessary to heat and remelt to expel the dissolved oxygen. 2.The medium can be incubated in ambient atmosphere if used in combination with a Brewer's lid replacing the standard Petri dish lid. Brewer described a special Petri dish cover that allowed surface cultivation of anaerobes and microaerophiles without the use of anaerobic equipment such as an anaerobic jar or pouch.
Formula, QC, and performance
- Formula / composition: originally developed by Brewer.
- Performance: specifications; Grams per litre of purified water.
Risk controls for routine use
- Warnings / precautions: For professional use only. Operators must be trained and have certain experience in the laboratory methods. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store the prepared medium at 2-8°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Acute Tox. 4, Acute Toxicity (Category 4), H302 Skin Sens. 1, Skin sensitization (Category 1); H317 Muta.
- Exposure controls: 8.1 Control parameters • Sodium thioglycolate (CAS No. 367-51-1) DNEL Limit value type: DNEL Consumer (systemic) Exposure route: Oral Exposure frequency: Long-term Limit value: 0.174 mg/m3 Limit value type: DNEL Consumer (systemic) Exposure route: Dermal Exposure frequency: Long-term Limit value: 19.3 ug/kg bw/day Limit value type: DNEL Consumer (systemic) Exposure route: Oral Exposure frequency: Long-term Limit value: 0.1 mg/kg bw/day Limit value type: DNEL worker (local) Exposure route:
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610320
- Catalog/document code matched: 610320, 2021
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/anaerobic-agar-brewer-for-microbiology-lab-testing