Product overview
Liofilchem Pseudomonas Agar F Culture Media 610309 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is pSEUDOMONAS AGAR F is a dehydrated medium used for isolation and differentation of Pseudomonas based on the formation of fluorescein. It is recommended by Unites States of Pharmacopoeia XXIII (1995) for use in the
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Where this product fits
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Result signals and confirmation
Pseudomonas aeruginosa appears on Pseudomonas Agar F as colonies surrounded by a yellow to greenish-yellow zone resulting from fluorescein production. If pyocianin is also synthesized, a bright green color is produced which fluoresces under UV light.
Method principle
The ratio of peptones in peptospecial is conducive of fluorescein production by Pseudomonas. These peptones contain phosphorus, which is stimulatory of fluorescin production. The addition of dipotassium phosphate increases the phosphorus content of the medium, thereby enhancing production of the fluorescent pigment. Magnesium sulfate provides essential ions for fluorescin production. The added glycerol acts as a souce of energy and contributes to the pigment production.
Bench workflow
- Preparation: Suspend 37,0 g of powder in 990 mL of distilled or deionized water. Add 10 mL of Glycerol Supplement (code 80021). Sterilize in autoclave at 121°C for 15 minutes. Aseptically dispense into final containers.
- Procedure: Specimens must first be isolated in pure culture on an appropriate medium. The isolate should be Gram-stained and examined to confirm that morphology is appropriate for Pseudomonas. Using a sterile loop inoculate the surface of the culture medium with several colonies. Incubate at 36± 1 °C for 18-24 hours. If the isolate fails to grow or grows slowly, reincubate at 28 ± 2 °C for 1-2 days and observe for growth and pigment production.
Controls and performance checks
- Formula / composition: (g/L) Peptospecial 20.0 Magnesium sulfate 1.5 Di-potassium phosphate 1.5 Agar 14.0 Final pH 7.2 ± 0.2
- Performance: of Microbial Limit Tests.
Storage, limits, and safety
- Warnings / precautions: The product is not classified as hazardous by current legislation and does not contain harmful substances in concentrations of ≥1%. The product is designed for in vitro diagnostic use and must be used only by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared media at 2-8 °C. WARNING and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610309
- Catalog/document code matched: 610309, 1995
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/pseudomonas-agar-f-for-microbiology-lab-testing