Laboratory use profile
Liofilchem Brettanomyces Agar Culture Media 163942 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is brettanomyces Agar is a selective medium used for microbial monitoring in the alcoholic beverage industry.Brettanomyces (or Dekkera) yeasts, including B. bruxellensis, B. lambicus, B. anomalus, B. custersianus, B. naardenensis, B. intermidius and B. nanus, are primarily known as wine and beer spoilage organisms. However, in some cases, Brettanomyces/Dekkera may play a positive role in a red wine’s flavor and bouquet and be an integral part of its character.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Use case and sample types
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Decision criteria after incubation
Brettanomyces/Dekkera spp produce yellow cream pinpoint colonies which change to green with increasing incubation time. The colour of the medium changes to yellow due to acid production. In addition, the detection of 4-ethylphenol by its phenolic smell, aids in the differentiation of Dekkera/Brettanomyces spp from other yeast species. Colony aspects should be observed by microscopy examination to check that microorganisms exhibit Brettanomyces morphology features.
Method logic
Casein peptone and malt extract provide nitrogen, amino acids, vitamins and minerals for organisms growth. Yeast extract is a source ofvitamins, particularly of group B. Glucose is the fermentable carbohydrate. Yeast nitrogen base provides growth factors for yeasts. Coumaric acid is the substrate for the production of 4-ethylphenol. Thiamine is a growth factor. Cyloheximide and chloramphenicol inhibit contaminating flora such as Saccharomyces cerevisiae. Ethanol improves recovery of Brettanomyces. Bromcresol green is the pH indicator. Agar is the solidifying agent.
Preparation and execution notes
- Procedure: Filter the sample through a sterile membrane (pore size 0.45 μm). Place the membrane onto the agar surface. Incubate at 25-30°C for up to 7 days.
Formula, QC, and performance
- Formula / composition: (per litre of purified water)Casein Peptone 5.0 gYeast Extract 3.0 gGlucose10.0 gMalt Extract 3.0 gYeast Nitrogen Base 3.0 gCoumaric Acid 0.1 gThiamine 0.02 gCycloheximide 0.01 gChloramphenicol 0.1 gBromocresol Green 0.02 gEthanol16 mlAgar18.0 gFinal pH 5.3 ± 0.2 at 25°C
- Quality control: 1.Control of general characteristics, label and print2.Sterility control7 days at 22 ± 2°C, in aerobiosis7 days at 35 ± 2°C, in aerobiosis3.Microbiological controlInoculum for productivity: 50-100 CFUInoculum for selectivity: 104-106 CFUIncubation Conditions: 5-7 days at 25-30°CMicroorganismGrowthDekkera bruxellensisATCC 36235GoodSaccharomyces cerevisiaeATCC 9763InhibitedTABLE OF SYMBOLS Batch code Do not reuse Manufacturer Use by Fragile, handle with care Catalogue number Temperature limitation Contains sufficient for <n> tests Caution, consult instructions for use LIOFILCHEM S.r.l.Via
Risk controls for routine use
- Warnings / precautions: The product contains hazardous substances and is classified as dangerous. It is recommended to consult the safety data sheet for its correct use. The product is designed for professional use only and must be used by properly trained operators.
- Storage / shelf life: CONDITIONS 10-25°C away from light, until the expiry date on the label. Eliminate if signs of deterioration or contamination are evident.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.REFERENCES1.Ocón E. et al. (2013) Comparison of culture media for the recovery of airborne yeast in wineries. Letters in Applied Microbiology; 57(3): 241-8. 2.Morneau AD, JM Zuehlke and CG Edwards (2011) Comparison of media formulations used to selectively cultivate Dekkera⁄Brettanomyces. Letters in Applied Microbiology; 53:460-5. 3.Rodrigues N. et al. (2001) Development and use of a new medium to detect yeasts of the genera Dekkera/Brettanomyces.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classif ication in any hazard class in accordance to Regula tion (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Ethyl alcol CAS: 64-17-5 TWA (Italy) - Short-term value: 1884 mg/mQ, 1000 ppm A3 PEL (U.S.A.) - Long-term value: 1900 mg/mQ, 1000 ppm REL (U.S.A.) - Long-term value: 1900 mg/mQ, 1000 ppm TLV (U.S.A.) - Short-term value: 1880 mg/mQ, 1000 ppm DNEL CAS: 64-17-5 Ethyl alcol Dermal DNEL (systemic chronic workers-effects) 343 mg/kg By inhalation DNEL (acute local effects workers) 19 mg/m3 DNEL (systemic chronic workers-effects) 950
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74163942
- Catalog/document code matched: 163942
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/brettanomyces-agar-for-microbiology-lab-testing