Product overview
Liofilchem TRIPLE SUGAR Iron Agar (TSI) Culture Media 610350 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is triple Sugar Iron (TSI) Agar is a differential medium used for the identification of Enterobacteriaceae on the basis of four reactions, i.e. fermentation of lactose, glucose and sucrose and production of hydrogen sulphide. TSI Agar is formulated according to ISO 6579 and 6785.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Where this product fits
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Result signals and confirmation
Examine for carbohydrates fermentation, gas production and hydrogen sulfide production. Carbohydrates fermentation results in an acidification of the medium that turns yellow: •an alkaline slant-acid butt (red/yellow) indicates fermentation of glucose only; •an acid slant-acid butt (yellow/yellow) indicates fermentation of glucose, lactose and/or sucrose; •an alkaline slant-alkaline butt (red/red) indicates that the organism being tested is a non-fermenter. Gas production from glucose is indicated by splitting and cracking of the medium.
Method principle
Meat extract and peptone provide amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Yeast extract is a source of vitamins, particularly of B-group. Sodium chloride maintains the osmotic balance of the medium. Lactose, sucrose and glucose are the fermentable carbohydrates. Sodium thiosulfate is reduced to hydrogen sulphide which in turn reacts with ferric ions yielding a black precipitate. Phenol red is the pH indicator. Agar is the solidifying agent.
Bench workflow
- Preparation: Dehydrated mediumSuspend 65.6 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Medium in bottles Melt the content of the bottle in a water bath at 100°C (loosing the cap partially removed) until completely dissolved. Then screw the cap and check the homogeneity of the dissolved medium, if it is the case turning the bottle upside down. Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into final containers.
- Procedure: Touch an isolated colony with either a sterile needle or loop. Inoculate by streaking over the surface of the slope and stabbing in the butt of the tube. Incubate aerobically (with caps loosened) at 37°C for 24 hours. Do not incubate longer than 24 hours because the acid reaction in the slant of lactose and sucrose fermenters may revert to an alkaline reaction.
Controls and performance checks
- Formula / composition: (g/l) Meat Extract 3.0 Peptone 20.0 Yeast Extract 3.0 Sodium Chloride 5.0 Lactose 10.0 Sucrose 10.0 Glucose 1.0 Sodium Thiosulfate 0.3 Ferric Ammonium Citrate 0.3 Phenol Red 0.024 Agar 13.0 Final pH 7.4 ± 0.2 at 25°C
- Quality control: Plates are inoculated with the microbial strains indicated in the QC table. Inoculum for productivity: ≤100 CFU. Incubation conditions: aerobically at 35 ± 2°C for 18-24 hours. QC Table. Microorganism Growth Slant Butt Gas H2S Escherichia coli ATCC 25922 Good Yellow Yellow + − Proteus mirabilis ATCC 12453 Good Red Yellow − + Pseudomonas aeruginosaATCC 27853 Good Red Red − − Salmonella typhimuriumATCC 14028 Good Red Yellow ± + Shigella flexneri ATCC 12022 Good Red Yellow − − WARNING AND
Storage, limits, and safety
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for professional use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store bottles, tubes and prepared plates at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The produ ct must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610350
- Catalog/document code matched: 610350, 2016, 6579, 6785
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/triple-sugar-iron-agar-tsi-for-microbiology-lab-testing