Product overview
Liofilchem AMIES Transport Medium (w/o CHARCOAL) Culture Media 610191 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is semi-solid medium for transport of clinical specimens and other microorganisms.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Where this product fits
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Result signals and confirmation
of dehydrated medium: beige, free-flowing, homogeneous.
Method principle
Amies Transport Medium w/o Charcoal provides a reduced environment due to the presence of sodium thioglycollate and the small amount of agar. The Chloride salts supply essential electrolytes for transport and osmotic balance. Phosphates act as a buffer system. Sodium thioglycollate lowers the redox potential of the medium, thereby allowing a better conservation of anaerobic bacteria. The survival of bacteria in a transport medium depends on various factors such as bacteria type and concentration in the specimen, transport medium formulation, the temperature and duration of transport, and inoculation to appropriate culture media within 24 hours. Optimal growth and typical morphology can only be expected if direct inoculation and appropriate cultivation are followed.
Bench workflow
- Preparation: Suspend 9.8 g of powder in 1 liter of distilled or deionized water. Heat to boiling, shake until completely dissolved and dispense into final containers as appropriate. Sterilize in autoclave at 121°C for 15 minutes.
- Materials required: Standard microbiological supplies and equipment such as: inoculating loop, pipettes, culture media, swab,
- Procedure: After collecting the clinical specimen with a swab, insert one third of the swab into the center of the medium. Then cut the stick and screw down the cap to force the swab to the bottom of the medium. Make sure the cap is screwed firmly on the bottle and keep cool during the transport period. © Liofilchem - Amies Transport Medium w/o Charcoal- Rev.1 / 16.09.2024 Page 2 of 7
Controls and performance checks
- Formula / composition: Composition (g/l) Sodium Chloride 3.0 Calcium Chloride 0.1 Potassium Chloride 0.2 Magnesium Chloride 0.1 Monopotassium Phosphate 0.2 Disodium Phosphate 1.15 Sodium Thioglycollate 1.0 Agar 4.0 Final pH = 7.2±0.2 at 25 °C
- Quality control: organisms. SPECIMENS Collect specimens with sterile swabs and transport to the laboratory. Process each specimen using procedures appropriate for that sample. Refer to specific guidelines for more detailed information.
Storage, limits, and safety
- Limitations: • Some growth of contaminants may also occur during the long period of transport. • After transportation, the specimen should be inoculated in the proper medium as soon as possible. For optimum results, the time lapse between sample collection and inoculum onto culture medium should be reduced to the minimum. • It may not be suitable for the transport of fastidious organisms. • Gonococci survive well in Amies Transport Medium for at least 6 to 12 hours provided they are not exposed to temperature extremes.
- Warnings / precautions: 1) For in vitro diagnostic use (IVD). 2) For laboratory professional use only. 3) Operators must be trained and have certain experience. Please read the instructions carefully before using the product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. 4) Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. 5) Do not use if the product or packaging appears to be damaged. 6) Follow standard
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30°C, in a dry environment, tightly closed in its original container and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared tubes at 2-8 °C.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Acute tox 4, Oral (Categoria 4), H302 Skin Sens.1, Skin sensitization (Category 1); H317 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H302 H317 Harmful if swallowed May cause an allergic skin reaction Precautionary statement(s) P261 P264 P270 P272 Avoid breathing fume Wash hands thoroughly after
- Exposure controls: 8.1 Control parameters • Sodium thioglycolate (CAS No. 367-51-1) DNEL Limit value type : DNEL Consumer (systemic) SAFETY DATA SHEET (According to Regulation EC n° 1907/2006 and to Commission Regulation EU 2020/878) Revision: 3 / Revision Date: 17.04.2023 ©Liofilchem s.r.l. Via Scozia 64026, Roseto degli Abruzzi (TE) Italy - Tel +39 0858930745 - Fax +39 0858930330 MSDS Ref.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product m ust be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610191
- Catalog/document code matched: 610191, 2024
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/amies-transport-medium-w-o-charcoal-for-microbiology-lab-testing