Product overview
Liofilchem LETHEEN Broth Base MODIFIED ISO 21149 Culture Media 610328 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is lETHEEN BROTH BASE MODIFIED ISO 21149 is a dehydrated medium for the microbiological testing of cosmetics, prepared according ISO 21149.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Where this product fits
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Result signals and confirmation
Count the colonies on the plate, multiply for the diluition factor and express the result as UFC per g or mL of sample.
Method principle
Peptone, tryptone and meat extract provide nitrogen and other nutrient to support microbial growth. Yeast extract is a source of amino acids and vitamins of group B. Sodium chloride maintains the osmotic balance of the medium. Lecithin and sodium bisulfite aim to neutralize the preservatives commonly present in cosmetics.
Bench workflow
- Preparation: Suspend 37.8 g of powder in 1 liter of distilled or deionized water. Add 5 mL of Tween 80 (ref. 80031). Heat until completely dissolved. Dispense in final containers. Sterilize in autoclave at 121 °C for 15 minutes.
- Procedure: ! Inoculate 9 mL of LETHEEN BROTH BASE MODIFIED with 1 g or 1 mL of sample to test. Use larger volumes of broth if high levels of contamination are expected. ! Mix well to homogenize the suspension. ! Incubate at 32.5 ± 2.5 °C for at least 20 hours. ! Using a sterile pipette transfer 0.1 to 0.5 mL of suspension on a plate containing a nutritive medium and spread the sample onto the entire surface using a sterile loop. ! Incubate at 32.5 ± 2.5 °C for 48-72 hours.
Storage, limits, and safety
- Warnings / precautions: The product is not classified as hazardous by current legislation and does not contain harmful substances in concentrations of !1%. The product must be used only by properly trained operators.
- Storage / shelf life: 10-30°C away from light, until the expiry date on the label or until signs of deterioration or contamination are evident. Store the prepared medium at 2-8 °C. WARNING and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610328
- Catalog/document code matched: 21149, 610328
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/letheen-broth-base-modified-iso-21149-for-microbiology-lab-testing