Product and use case
Liofilchem E.M.B. Agar w Lactose + SUCROSE Culture Media 610140 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is selective medium for isolation of enteric Gram-negative bacteria from clinical specimens. This medium is intended as an aid in the diagnosis, requiring further tests to complete the diagnostic results.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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When this format is useful
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
What to read after incubation
At the end of the incubation period evaluate colonies characteristics as follows: Colonies of lactose fermenters are blue-black with or without a green metallic sheen. E. coli typically produces dark centered colonies with a green metallic sheen. Enterobacter spp. appear as brown to blue-black, mucoid colonies with no sheen. Colonies of non-lactose fermenting bacteria, such as species of Shigella and Salmonella, are colorless and translucent.
Selective or enrichment logic
The eosin dye inhibits growth of gram-positive bacteria and combines with the methylene blue indicator to produce a color change whenever lactose or sucrose are fermented. This medium does not allow discrimination between which carbohydrate is fermented. For example, Yersinia enterocolitica, which ferments sucrose, but not lactose, will produce the same purple-black colony as lactose-fermenting bacteria.
Sample handling workflow
- Preparation: Dehydrated medium Suspend 38.0 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until dissolved completely. Sterilize in autoclave at 121°C for 15 minutes. Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into Petri dishes.
- Materials required: Standard microbiological supplies and equipment such as: autoclave, sterile Petri plates, test tubes, inoculating loops, swabs, incubator,
- Procedure: Inoculate a plate with the sample and streak for isolation. Incubate plates, protected from light, at 35 ± 2°C for 18-24 hours. If negative after 24 hours, reincubate an additional 24 hours.
Technical verification points
- Formula / composition: * (g/litre) Peptone 10.0 Lactose 5.0 Sucrose 5.0 Potassium phosphate bibasic 2.0 Eosine Y 0.4 Methylene blue 0.066 Agar 15.0 Final pH 7.1 ± 0.2 *Adjusted and/or supplemented as required to meet
- Quality control: organisms. SPECIMENS Clinical specimens should be sampled at the acute stage, before antimicrobial therapy (where possible) and examined as soon as possible after collection. Good laboratory practices for collection, transport and
Storage, hazards, and disposal
- Limitations: Invalid results can be caused by poor specimen quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the principles of the procedures, including its
- Warnings / precautions: 1) For in vitro diagnostic use (IVD). 2) For laboratory professional use only. 3) Operators must be trained and have certain experience. Please read the instructions carefully before using the product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. 4) Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. 5) Do not use if the product or packaging appears to be damaged. © Liofilchem - EMB Agar - Rev.0 / 29.08.2025 Page 3 of 7 6) Follow standard
- Storage / shelf life: of the clinical specimens should be applied. Refer to specific guidelines for more information about specimen collection and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610140
- Catalog/document code matched: 610140, 2025
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/e-m-b-agar-w-lactose-sucrose-for-microbiology-lab-testing