Product and use case
Liofilchem Violet Red Bile Agar w Lactose, Glucose Culture Media 610336 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is violet Red Bile Agar w Lactose, Glucose is a medium used for the selective isolation and differentiation of bile-tolerant Gram-negative bacteria in food, water and other materials.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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When this format is useful
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
What to read after incubation
Select plates containing less than 150 colonies. Count characteristic pink to red colonies (with or without precipitation halo).
Selective or enrichment logic
Pancreatic digest of gelatin provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Yeast extract is a source of vitamins, particularly of B-group. Lactose and glucose are fermentable carbohydrates. Sodium chloride maintains the osmotic balance of the medium. Bile salts and crystal violet are selective agents effective against Gram-positive cocci. Neutral red is the pH indicator. Agar is the solidifying agent.
Sample handling workflow
- Preparation: Suspend 51.5 g of powder in 1 liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Sterilize at 118°C for 15 minutes. Cool up to 45-50°C. Pour in Petri dishes.
- Procedure: Use a suitable diluent such as Buffered Peptone Water (ref. 24099) to prepare the sample. To resuscitate bacteria, a progressive enrichment is recommended in Tryptic Soy Broth (ref. 24444) for 2-5 h at 20-25°C and subsequently in EE Broth-Mossel (ref. 24096) for 24-48 h at 35 ± 2°C. Otherwise, inoculate sample dilutions directly in EE Broth-Mossel and incubate at 35 ± 2°C for 18-24 hours.Subculture to Violet Red Bile Agar w Lactose, Glucose by pour plating or spread plating method. Incubate aerobically at 35 ± 2°C for 18-24 hours
Technical verification points
- Formula / composition: (g/l)Pancreatic Digest of Gelatin 7.0Yeast Extract 3.0Lactose10.0Glucose10.0Sodium Chloride 5.0Bile Salts No. 3 1.5Crystal Violet 0.002Neutral Red 0.03Agar15.0Final pH 7.4 ± 0.2 at 25°C
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: 50-100 CFUInoculum for selectivity: 104-106 CFUIncubation Conditions: 18-24 h at 35 ± 2°C, in aerobiosisMicroorganismGrowthColony colorEscherichia coliATCC 8739GoodPink-redSalmonella TyphimuriumATCC 14028GoodPink-redPseudomonas aeruginosaATCC 9027GoodColorlessStaphylococcus aureusATCC 6538Inhibited---TABLE OF SYMBOLS Batch code Do not reuse Manufacturer Use by Fragile, handle withcare Catalogue number Temperature limitation Contains sufficientfor <n> tests Caution, consult instructions for
- Performance: testing of culture media. 2.European Pharmacopoeia 6.5 (2009) 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. 3.United States Pharmacopoeia 32 NF 27 (2009) <62> Microbiological examination of non-sterile products: Test for specified microorganisms. 4.Japanese Pharmacopoeia 4.05 (2008) Microbiological examination of non-sterile products: Test for specified microorganisms. 5.ISO 21528-1:2004. Microbiology of food and animal feeing stuffs − Horizontal method for the detection and enumeration of Enterobacteriaceae − Detection and enumeration by MPN
Storage, hazards, and disposal
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for professional use only and must be used by properly trained operators.
- Storage / shelf life: AND TRANSPORT CONDITIONSThe powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.REFERENCES1.EN ISO 11133:2014. Microbiology of food, animal feed and water −
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610336
- Catalog/document code matched: 610336
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/violet-red-bile-agar-w-lactose-glucose-for-microbiology-lab-testing