Product overview
Liofilchem Glucose OF Medium ISO 21528 Culture Media 610388 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is glucose OF medium is a semisoild medium used for the identification of gram-negative bacteria on the basis of their ability to oxidize or ferment glucose. This medium complies with ISO 21528:2017 for confirmation of Enterobacteriaceae from food, animal feeding stuffs and environmental samples.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Where this product fits
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Result signals and confirmation
If a yellow color develops throughout the medium the fermentation test is positive. According to ISO 21528, colonies that are oxidase-negative and glucose-positive are confirmed as Enterobacteriaceae.
Method principle
Enzymatic digest of casein provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Glucose is the fermentable carbohydrate. Sodium chloride maintains the osmotic balance of the medium. Dipotassium phosphate acts as buffer agent. Bromthymol blue is the pH indicator. Agar is the solidifying agent.
Bench workflow
- Preparation: Dehydrated medium Suspend 20.38 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. Dispense into tubes. Sterilize in autoclave at 121°C for 15 minutes.
- Procedure: Just before use, heat the medium in boiling water or flowing steam for 15 min to remove oxygen, then cool rapidly to the incubation temperature. Using a wire, inoculate tubes by stabbing colonies into the medium. Overlay with minimal 1 cm of sterile mineral oil (Vaseline Oil Droppers, ref. 87006). Incubate at 37°C for 24 hours.
Controls and performance checks
- Formula / composition: (g/l) Enzymatic Digest of Casein 2.0 Glucose 10.0 Sodium Chloride 5.0 Dipotassium Hydrogen Phosphate 0.3 Bromthymol Blue 0.08 Agar 3.0 Final pH 6.8 ± 0.2 at 25°C
- Quality control: The medium is inoculated with the microbial strains indicated in the QC table. Incubation conditions: 37 ± 1°C for 24 ± 2 hours. QC Table. Microorganism Growth Fermentation test Escherichia coli WDCM 00012 Good Positive (+), yellow color Pseudomonas aeruginosaWDCM 00025 Good Negative (−), yellow color only at the top of the tube WARNING AND
Storage, limits, and safety
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for professional use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store tubes at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610388
- Catalog/document code matched: 21528, 610388, 2017
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/glucose-of-medium-iso-21528-unit-500-g