Product overview
Liofilchem MIDDLEBROOK 7H10 Agar Base Culture Media 611022 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is solid medium used for the selective isolation of Mycobacterium spp., especially Mycobacterium tuberculosis, from clinical specimens as well as for the cultivation of pure cultures of mycobacteria. This medium is intended as an aid in the diagnosis, requiring additional tests to complete the diagnostic results.
This product fits laboratories that culture or support workups for mycobacteria, where slow growth, selective recovery, incubation duration, and contamination control all affect the usefulness of the final result.
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Where this product fits
Mycobacteria workflows need a medium choice that accounts for slow growth, long incubation, contamination pressure, and careful colony review. The method should define how often plates are examined, what colony features matter, and which follow-up identification steps are required.
Result signals and confirmation
Examine weekly for growth, pigment production and colony morphology. Carry out identification tests according to established laboratory procedures.
Method principle
Glutamic acid, sodium citrate, pyridoxine, biotin and ammonium sulfate supply growth factors. Ferric ammonium citrate, magnesium sulfate, calcium chloride, zinc sulfate and copper sulfate are sources of trace ions. Phosphates help maintaining the pH of the medium. Malachite green is the selective agent inhibiting the contaminant microbial flora. Malachite green serves as pH indicator as well. Agar is the solidifying agent. Glycerol and glucose are energy sources. Sodium chloride maintains the osmotic equilibrium. Albumin protects the tubercle bacilli against toxic agents. Catalase destroys toxic peroxides that may be present in the medium. Oleic acid is a fatty acid utilized in the mechanism of mycobacteria.
Bench workflow
- Preparation: Dehydrated medium. Suspend 19.5 g of powder in 900 ml of distilled or deionized water containing 5 ml of Glycerol supplement. Heat to boil until completely dissolved. Autoclave at 121 °C for 15 minutes. Cool to 45-50 °C and aseptically add 100 ml Middlebrook 7H10 supplement. Mix well and pour into sterile final containers (this medium is prepared in slant tubes or plates).
- Materials required: Standard microbiological supplies and equipment such as: Autoclave, sterile Petri plates, test tubes, inoculating loops, swabs, incubator,
- Procedure: Inoculate processed specimen onto the medium. Process clinical specimens as soon as possible. Concentration of samples (for example centrifugation) may be required. Depending on the specimen type and method used, it may be necessary to go through one or more of the following stages before culture: homogenization, digestion/ decontamination, and neutralization. Keep plates and tubes shielded from light and incubate at 35 ± 2°C for up to 8 weeks in aerobic atmosphere enriched with 5-10% carbon dioxide. For more detailed information, consult appropriate guidance.
Controls and performance checks
- Formula / composition: * (Per Litre of Purified Water) OADC: Oleic acid, Albumin, Dextrose, Catalase. *Adjusted and/or supplemented as required to meet
- Quality control: organisms. SPECIMENS Specimens submitted for mycobacterial culture fall into two categories: specimens normally contaminated with resident flora, and specimens from normally sterile sites. Contaminated specimens require a decontamination step before culture to reduce the likelihood of overgrowth by organisms other than mycobacteria. Specimens should be obtained before antimicrobial therapy (where possible) and promptly delivered to the laboratory for examination. Refer to specific guidelines for more detailed information.
- Performance: specifications. Complete medium Base + Supplements Middlebrook 7H10 Agar Base + Middlebrook 7H10 (OADC) Supplement + Glycerol Supplementation of the agar base is required to obtain mycobacterial growth.
Storage, limits, and safety
- Limitations: Negative culture results do not rule out active infection of mycobacteria. Some factors of unsuccessful cultures are: - The specimen was not representative of the infectious material, e.g. saliva instead of sputum. - The mycobacteria were destroyed during digestion and decontamination of the specimen. - Gross contamination interfered with the growth of mycobacteria. - Proper aerobic conditions and increased CO2 tension were not provided during incubation.
- Warnings / precautions: 1) For in vitro diagnostic use (IVD). 2) For laboratory professional use only. 3) Operators must be trained and have certain experience. Please read the instructions carefully before using the product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. 4) Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. 5) Do not use if the product or packaging appears to be damaged. 6) Follow standard
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store slant tubes and prepared plates at 2-8 °C.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards Results of PBT and vPvB assessment • PBT: Not applicable. • vPvB: Not applicable 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74611022
- Catalog/document code matched: 611022, 2023
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/middlebrook-7h10-agar-base-for-microbiology-lab-testing