Product and use case
Liofilchem Brilliant Green Agar w/ Sulfapyridine Culture Media 610394 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is brilliant Green Agar w/ Sulfapyridine is a selective medium used for the isolation of Salmonella spp, other than S. Typhi and S. Paratyphi, from food, animal feed, and other materials.
This product is relevant to food safety and environmental monitoring programs where Salmonella screening is part of raw material, finished product, or hygiene verification testing.
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When this format is useful
This product fits screening programs where a negative or presumptive result can affect raw-material release, finished-product hold decisions, or hygiene investigations. The lab should connect the medium to its enrichment sequence, confirmation method, and written rule for when suspect colonies move forward.
What to read after incubation
Typical Salmonella colonies on Brilliant Green Agar w/ Sulfapyridine appear as red to pink-white with red zones. The red coloration of the medium indicates that lactose or sucrose was not utilized.Lactose or sucrose fermenting microorganisms not completely inhibited will produce yellow to yellow-green colonies with a yellow-greenor green halo.Escherichia coli may be partially inhibited and present as yellow to yellow-green colonies surrounded by a green halo.Shigella spp.
Selective or enrichment logic
Enzymatic digest of animal tissue and enzymatic digest of casein provide amino acids, nitrogen, carbon, minerals, vitamins and other nutrients for organisms growth. Yeast extract is a source of vitamins, particularly of B-group. Sodium chloride maintains the osmotic balance of the medium. Lactose and sucrose are the fermentable carbohydrates. Brilliant green and sulfapyridine are selective agents, inhibiting Gram-positive bacteria and many Gram-negative organisms, except Salmonella spp. Phenol red is the pH indicator. Agar is the solidifying agent.
Sample handling workflow
- Preparation: Suspend 59 g of powder in 1 liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Sterilize at 121°C for 15 minutes. Cool up to 45-50°C. Mix well and pour in Petri dishes.
- Procedure: Inoculate Brilliant Green Agar w/ Sulfapyridine from a selective enrichment broth, e.g. Tetrathionate Broth (ref. 24451) or Selenite Cystine Broth (ref. 24510), or by direct streaking of the sample onto the plate. Incubate the plates aerobically at 35 ± 2°C for 18-24 h. If growth is not observed, extend the incubation for an additional 24 h. NOTE:For best results in isolating all enteric pathogens, a less selective medium, such as MacConkey Agar (ref. 10029) or Hektoen Enteric Agar (ref. 10043), should be used simultaneously.
Technical verification points
- Formula / composition: (g/l)Enzymatic Digest of Animal Tissue 5.0Enzymatic Digest of Casein 5.0Yeast Extract 3.0Sodium Chloride 5.0Lactose10.0Sucrose10.0Brilliant Green 0.0125Phenol Red 0.08Sodium Sulfapyridine 1.0Agar20.0Final pH 6.9 ± 0.2 at 25°C
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: 50-100 CFUInoculum for selectivity: 104-106 CFUIncubation Conditions: 18-24 h / 35 ± 2°CMicroorganismGrowthSpecificationSalmonella TyphimuriumATCC 14028 GoodRed to pink-white colonies with a red haloEscherichia coliATCC 25922Partially inhibitedYellow to yellow-green colonies with a green haloEnterococcus faecalisATCC 29212Inhibited---Proteus mirabilisATCC 12453Inhibited---TABLE OF SYMBOLS Batch code Keep away from Sunlight Manufacturer Use by Fragile, handle withcare Catalogue number
Storage, hazards, and disposal
- Warnings / precautions: The product contains hazardous substances and is classified as dangerous. It is recommended to consult the safety data sheet for its correct use. The product is designed for professional use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force. LIOFILCHEM S.r.l.Via Scozia, Zona Ind.le - 64026, Roseto degli Abruzzi (TE) - ITALYTel +39 0858930745 Fax +39 0858930330 www.liofilchem.com TECHNICAL SHEETTS 610394Rev.0 / 06.11.2019Page 2 of 3REFERENCES•Marshall, R. T. (ed.). Standard methods for the examination of dairy products, 16th ed., American Public Health Association, Washington, D.C. •Vanderzant, C., and D. F. Splittstoesser (eds.). Compendium of methods for the microbiological examination of foods, 3rd ed.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610394
- Catalog/document code matched: 610394
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/brilliant-green-agar-w-sulfapyridine-for-microbiology-lab-testing