Laboratory use profile
Liofilchem Campylobacter Blood FREE Medium Base Culture Media 610130 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is campylobacter Blood Free Medium Base is a selective medium used with supplements for the isolation and enumeration of Campylobacter spp from food, environmental samples and clinical specimens. The complete medium, also known as modified charcoal cefoperazone deoxycholate agar (mCCDA), is formulated according to the APHA and ISO 10272 and was developed to replace blood with charcoal, ferrous sulfate and sodium pyruvate.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Use case and sample types
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Decision criteria after incubation
Examine the plates for typical colonies of Campylobacter spp which appear greyish, flat and moist, often with a metallic sheen and a tendency to spread. Other form of colonies may occur. For confirmation of Campylobacter spp, subculture suspected colonies to Columbia Blood Agar plates (ref. 11025) and examine pure cultures for morphology, motility, microaerobic growth at 25°C, aerobic growth at 41.5°C and oxidase activity.
Method logic
Meat extract, enzymatic digest of animal tissues and enzymatic digest of casein provide amino acids, nitrogen, carbon, minerals, vitamins and other nutrients for organisms growth. Sodium chloride maintains the osmotic balance of the medium. Charcoal absorbs toxic compounds and metabolites. Sodium deoxycholate inhibits most Gram-positive bacteria. Ferrous sulfate and sodium pyruvate are oxygen scavengers. Agar is the solidifying agent.Supplementation with Campylobacter CCDA Supplement (ref. 81037), containing Cefoperazone and Amphotericin B, inhibits the accompanying microbial flora.
Preparation and execution notes
- Preparation: Suspend 45.5 g of powder in 1 liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Sterilize at 121°C for 15 minutes. Cool up to 45-50°C. Aseptically, add rehydrated content of 2 vials (10 ml) of Campylobacter CCDA Supplement. Mix well. Pour in Petri dishes.
- Procedure: Inoculate the plates by directly spreading the sample material over the agar surface (*). Incubate at 41.5°C for 40-48 hours in a microaerobic atmosphere (approximately 5-6% oxygen, 3-10% CO2 and 84-85% nitrogen).* ISO 10272 recommends to perform a first enrichment step in Bolton Broth (ref. 470340) prior to inoculate the mCCDA.
Formula, QC, and performance
- Formula / composition: (g/l)Meat Extract10.0Enzymatic Digest of Animal Tissues10.0Sodium Chloride 5.0Charcoal 4.0Enzymatic Digest of Casein 3.0Sodium Deoxycholate 1.0Iron(II) Sulfate 0.25Sodium Pyruvate 0.25Agar12.0Final pH 7.4 ± 0.2 at 25°C
Risk controls for routine use
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for in vitro diagnostic use only and must be used by properly trained operators.
- Storage / shelf life: AND TRANSPORT CONDITIONSThe powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610130
- Catalog/document code matched: 610130, 10272
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/campylobacter-blood-free-medium-base-for-microbiology-lab-testing