Laboratory use profile
Liofilchem Violet Red Bile Lactose Agar Culture Media 610058 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is violet Red Bile Lactose Agar is a selective medium used for the isolation and enumeration of coliform bacteria in food, water and other materials of sanitary importance, according to APHA and ISO 4832.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Use case and sample types
Use this product where indicator organisms are part of a release, trend, or sanitation decision. The practical value comes from separating countable or presumptive target reactions from background flora so the analyst can document whether the sample meets the site limit.
Decision criteria after incubation
Select plates containing 10-150 colonies. Count the purplish-red colonies with a diameter of at least 0.5 mm. Atypical colonies (e.g. smaller size) and all colonies derived from milk products should be confirmed by using Brilliant Green Lactose Bile Broth 2% (ref. 20102).
Method logic
Enzymatic digest of animal tissues provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Yeast extract is a source of vitamins, particularly of B-group. Lactose is the fermentable carbohydrate. Sodium chloride maintains the osmotic balance of the medium. Bile salts and Crystal violet are selective agents effective against Gram- positive cocci. Neutral red is the pH indicator. Agar is the solidifying agent.
Preparation and execution notes
- Preparation: Dehydrated medium Suspend 40.5 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. DO NOT AUTOCLAVE. Medium in tubes/bottles Melt the content of the tube/bottle in a water bath at 100°C (loosing the cap partially removed) until completely dissolved. Then screw the cap and check the homogeneity of the dissolved medium, if it is the case turning the tube/bottle upside down. Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into Petri dishes.
- Procedure: 1.Perform serial dilutions of the test sample in order to achieve a colony count of between 10 and 150 colonies per plate. Use a suitable diluent such as Buffered Peptone Water (ref. 24099) or Maximum Recovery Broth (ref. 20071). 2.Inoculate the medium by pour plating or spread plating method. 3.Incubate aerobically at 30°C or 37°C, depending on the organisms under study, for 24 ± 2 hours. For environmental hygiene monitoring, use a swab and the sampling template 10x10 (ref. 96762) to sample a well defined area of the test surface. Then, inoculate the medium by streaking the swab over the plate. Otherwise, RODAC plates can be directly used for surface sampling by firmly pressing the agar medium against the test area for a few seconds.
Formula, QC, and performance
- Formula / composition: (g/l) Enzymatic Digest of Animal Tissues 7.0 Yeast Extract 3.0 Lactose 10.0 Sodium Chloride 5.0 Bile Salts 1.5 Neutral Red 0.03 Crystal Violet 0.002 Agar 14.0 Final pH 7.4 ± 0.2 at 25°C
- Quality control: Plates are inoculated with the microbial strains indicated in the QC table. Inoculum for productivity: 50-100 CFU. Inoculum for selectivity: 104-106 CFU. Inoculum for specificity: 103-104 CFU. Incubation conditions: aerobically at 30 ± 1°C for 24 ± 2 hours. QC Table. Microorganism Specification Escherichia coli WDCM 00012 Good growth, purplish-red colonies with or without precipitation halo Enterococcus faecalis WDCM 00009 Inhibition Pseudomonas aeruginosa WDCM 00025 Colorless to beige colonies WARNING AND
Risk controls for routine use
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for professional use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store tubes and prepared plates at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610058
- Catalog/document code matched: 610058, 2015, 4832
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/violet-red-bile-lactose-agar-for-microbiology-lab-testing