Product overview
Liofilchem Mannitol MOTILITY Test Medium Culture Media 610235 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is mANNITOL MOTILITY TEST AGAR is a semisolid medium used for the detection of motility of Enterobacteriaceae.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Where this product fits
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Result signals and confirmation
The expected result depends on the validated method for surface hygiene and environmental monitoring. The analyst should document the observation that drives the decision, such as colony appearance, count, inhibition pattern, growth/no-growth response, or need for confirmatory testing.
Method principle
Bacterial motility can be observed directly from examination of the tubes following incubation. Growth spreads out from the line of inoculation if the organism is motile. Highly motile organisms provide growth throughout the tube. Growth of non-motile organisms only occurs along the stab line.
Bench workflow
- Preparation: Suspend 28.0 g of powder in 1 litre of distilled or deionized water. Heat to boiling and shake until completely dissolved. Dispense in final tubers. Sterilize at 121°C for 15 minutes.
- Procedure: Inoculate tubes with a pure culture by stabbing the center of the column of medium to greater than half the depth. Incubate tubes for 24-48 hours at 35 ± 2°C in an aerobic atmosphere.
Controls and performance checks
- Formula / composition: (g/L) Peptone 20.0 Mannitol 2.0 Potassium Nitrate 1.0 Phenol Red 0.04 Agar 5.0 Final pH 7.3 ± 0.2
Storage, limits, and safety
- Warnings / precautions: The product is not classified as hazardous by current legislation and does not contain harmful substances in concentrations of ≥1%. The product is designed for In vitro diagnostic use and must be used only by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared media at 2-8°C. WARNING and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610235
- Catalog/document code matched: 610235
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/mannitol-motility-test-medium-for-microbiology-lab-testing