Product and use case
Liofilchem Clostridium DIFFICILE Agar Base Culture Media 610115 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is clOSTRIDIUM DIFFICILE AGAR is a medium used for the isolation of Clostridium difficile.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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When this format is useful
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
What to read after incubation
C. difficile grows with whitish opaque colonies of 4-6mm diameter irregular.
Selective or enrichment logic
Proteose peptone provides nitrogen, vitamins, minerals and amino acid essential for growth. Fructose is the fermentable carbohydrateused to facilitate the recovery and growth of C. difficile. Potassium dihydrogen phospahate and disodium hydrogen phosphate act asbuffering system. Magnesium sulphate is source of magnesium ions essential for several enzymatic reactions and DNA duplication.Sodium chloride maintains the osmotic balance of the medium. This basal medium needs to be added with horse blood that suppliesessential growth factors and selective agents to inhibit the growth of most of the microorganisms present in fecal sample other than C.difficile.
Sample handling workflow
- Preparation: Suspend 34.6 g of powder in 500 ml of distilled water. Heat until completely dissolved. Autoclave at 121°C for 15 minutes. Cool to 45-50°C. Aseptically add 1 vial of CLOSTRIDIUM difficile Supplement (ref. 81007) previously reconstituted with 2 ml of sterile distilled waterand 35 ml of HORSE BLOOD DEFIBRINATED (ref. 83395). Mix gently and dispense in Petri dishes.
- Procedure: Direct inoculum of fecal samplesLightly inoculate the medium with fecal sample spreading part of the original inoculum in order to obtain well separated colonies.Incubate the plates at 36+/-1°C for 18-24 hours anaerobically. Treatment for alcohol shockMix equal part of absolute ethyl alcohol and the fecal specimen. Homogenize using a vortex mixer. Leave at room temperature of 1hour. Inoculate the medium and incubate the plates at 36+/-1°C for 18-24 hours anaerobically.
Technical verification points
- Formula / composition: (g/l)Proteose Peptone40.0Fructose 6.0Disodium Hydrogen Phosphate 5.0Potassium Dihydrogen Phospahate 1.0Magnesium Sulphate 0.1Sodium Chloride 2.0Agar15.0Final pH 7.4 ± 0.2 at 25°C
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: 10-100 CFU/mlInoculum for selectivity: 104-105 CFU/mlInoculum for specificity: !104 CFU/mlIncubation conditions:18-24 h at 36 ± 1°C anaerobicallyMicroorganismGrowthEscherichia coli ATCC 25922 InhibitedClostridium difficile ATCC 9869GoodTABLE OF SYMBOLS Batch code In vitrodiagnosticmedical device Manufacturer Use by Fragile, handlewith care Cataloguenumber Temperaturelimitation Contains sufficientfor <n> tests Consult instructionfor use Do not reuse Keep awayfrom heatsources LIOFILCHEM
Storage, hazards, and disposal
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product must be usedonly by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use itbefore the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8°C away fromlight.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to national and local regulations in force.REFERENCES1.Levett (1985) J. Clin. Pathol. 38: 233-234. 2.Barlett,. J.G. et al. (1978) N. Eng. J. Med., 298 , 531. 3.Boriello, S.P. et al (1981) J. Antimicrob. Chemother. 7 Supp. A. 53-62. 4.George, R.H. et al (1976) J. Clin. Microbiol. 6, 214-219.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610115
- Catalog/document code matched: 610115
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/clostridium-difficile-agar-base-for-microbiology-lab-testing