Laboratory use profile
Liofilchem TRYPTONE Glucose Extract Agar Culture Media 610089 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is tRYPTONE GLUCOSE EXTRACT AGAR is used for cultivating and enumerating microorganisms in water and dairy products.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Use case and sample types
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Decision criteria after incubation
Follow recommended procedures for counting colonies and reporting results 1. Because bacteria found in bottled water demonstrate a prolonged lag phase during adaptation to growth on TRYPTONE GLUCOSE EXTRACT AGAR, extended incubation beyond 48 hours may be required1.
Method logic
Beef extract, tryptone provide nitrogen, carbon, sulphur and other essential growth factors. Dextrose is the source of energy. Agar is the solidifying agent.
Preparation and execution notes
- Preparation: Suspend 24.0 g of powder in 1 litre of distilled or deionized water. Heat to boiling and shake until completely dissolved. Sterilize at 121°C for 15 minutes. Dispense in final containers.
- Procedure: Consult the reference for information regarding the processing and inoculation of bottled water samples. 1 Inoculate 1 mL samples of appropriate dilutions of the test sample into sterile Petri dishes and molten, cooled TRYPTONE GLUCOSE EXTRACT AGAR is added followed by gentle mixing to distribute the sample dilution throughout the agar. Incubate at 32+/-1°C, or as specified in standard methods (35++/-0,5°C for at least 72 hours)1 .
Formula, QC, and performance
- Formula / composition: (g/L) Beef extract...................................................................... 3.0 Tryptone........................................................................... 5.0 Dextrose....………………………………………………....... 1.0 Agar.................................................................................. 15.0 Final pH 7.0 ± 0.2
Risk controls for routine use
- Warnings / precautions: The product is not classified as hazardous by current legislation and does not contain harmful substances in concentrations of ≥1%. The product must be used only by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30°C, in a dry environment, in its original container tightly closed until the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared media at 2-8°C. WARNING and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610089
- Catalog/document code matched: 610089
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/tryptone-glucose-extract-agar-for-microbiology-lab-testing