Product and use case
Liofilchem Pseudomonas Agar P Culture Media 610310 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is medium used with supplements for the isolation of Pseudomonas spp. from clinical specimens, meat and dairy products, water and environmental samples. This medium is intended as an aid in the diagnosis, requiring further tests to complete the diagnostic results.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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When this format is useful
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
What to read after incubation
Pseudomonas aeruginosa can grow on Pseudomonas Agar P to form colonies surrounded by a blue to green zone due to pyocyanin formation. The presence of pyocyanin can be confirmed using chloroform to extract the pigment. © Liofilchem - Pseudomonas Agar P - Rev.0 / 13.02.2024 Page 2 of 7
Selective or enrichment logic
Pancreatic digest of casein provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. The gelatin peptone is low in phosphorous to minimize the inhibitory action on pyocyanin production. Magnesium chloride and potassium sulfate are cationic salts, which act as activators for pyocyanin production. Agar is the solidifying agent. Glycerol added to the prepared medium, is an energy source. Pseudomonas aeruginosa is the only species of bacteria known to produce pyocyanin, a blue-green pigment which diffuses into the agar surrounding microorganism growth.
Sample handling workflow
- Preparation: Suspend 46.0 g of the powder in 990 ml of distilled or deionized water. Add 10 ml of Glycerol Supplement. Heat to boil, shaking frequently until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes. Alternatively, distribute into tubes before sterilization and let solidify in slanted position with a short slant.
- Materials required: Standard microbiological supplies and equipment such as: Autoclave, water bath, sterile Petri plates, test tubes, inoculating loops, swabs, incubator,
- Procedure: The sample consists of pure culture of bacteria. Inoculate the medium by streaking the sample over the agar surface. Incubate at 35 ± 2 °C for 18-24 hours. If necessary, extend incubation for 1-2 days.
Technical verification points
- Formula / composition: * (g/litre) Pancreatic Digest of Casein 20.0 Magnesium Chloride 1.4 Potassium Sulfate 10.0 Agar 15.0 Final pH 7.2 ± 0.2 at 25°C *Adjusted and/or supplemented as required to meet
- Quality control: organisms. SPECIMENS Clinical specimens should be sampled at the acute stage, before antimicrobial therapy (where possible) and examined as soon as possible after collection. Good laboratory practices for collection, transport and
Storage, hazards, and disposal
- Limitations: Invalid results can be caused by poor specimen quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the principles of the procedures, including its
- Warnings / precautions: 1) For in vitro diagnostic use (IVD). 2) For laboratory professional use only. 3) Operators must be trained and have certain experience. Please read the instructions carefully before using the product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. 4) Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. 5) Do not use if the product or packaging appears to be damaged. 6) Follow standard
- Storage / shelf life: of the clinical specimens should be applied. Refer to specific guidelines for more information about specimen collection and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610310
- Catalog/document code matched: 610310, 2024
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/pseudomonas-agar-p-for-microbiology-lab-testing