Product and use case
Liofilchem DICHLORAN GLYCEROL(DG18)Agar Base Culture Media 610238 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is dichloran Glycerol (DG18) Agar Base is a medium used with supplements for the selective isolation and enumeration of fungi in food.The complete medium conforms to ISO 21527-2 for microbiological examination of products that have a water activity less than or equalto 0.95 such as dry fruits, cakes, jams, dried meat, salted fish, grains, cereal products, flour, nuts, spices and condiments, etc.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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When this format is useful
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
What to read after incubation
Select plates containing <150 colonies/propagules/germs and count after 2 days and again after 5-7 days of incubation. Report theresults as CFU per g or per ml of sample allowing for the dilution factor.
Selective or enrichment logic
Enzymatic digest of casein provides amino acids, carbon, nitrogen, vitamins and minerals for organisms growth. Glucose is included as energy source. Monopotassium phosphate is a buffering agent. Magnesium sulfate provides divalent cations and sulfur. Dichloran is an anti-fungal agent incorporated into the medium to reduce colony diameters of spreading fungi easing the colony count. Inhibition of bacterial growth and restriction of spreading of more-rapidly growing moulds aids in the isolation of slow-growing fungi. Agar is the solidifying agent. Glycerol Supplement (ref. 80021) is included to lower the water activity of the medium and provide an additional carbon source.Chloramphenicol Supplement (ref. 81017) is added to inhibit the growth of accompanying bacterial flora.
Sample handling workflow
- Preparation: Suspend 31.5 g of powder in 1 liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Add 220 g glycerol (about 180 ml Glycerol Supplement ) and the reconstituted content of 2 vials (14 ml) of Chloramphenicol Supplement. Sterilize at 121°C for 15 minutes. Cool up to 45-50°C. Pour in Petri dishes.
- Procedure: According to ISO 21527-2, spread 0.1 ml of the the test sample or 0.1 ml of the initial suspension onto one plate of Dichloran Glycerol (DG18) Agar and 0.1 ml of the first decimal dilution over a second plate. Repeat inoculation with subsequent dilutions. Incubate plates aerobically at 25 ± 1°C for 5-7 days and up to 10 days if the presence of Xeromyces bisporus is suspected.
Technical verification points
- Formula / composition: (g/l)Casein Enzymatic Digest 5.0D-Glucose10.0Monopotassium Phosphate 1.0Magnesium Sulfate 0.5Dichloran 0.002Agar15.0Final pH 5.6 ± 0.2 at 25°C
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: 50-100 CFUInoculum for selectivity: 104-106 CFUIncubation Conditions: 5 days at 25 ± 1°C, in aerobiosisMicroorganismGrowthSaccharomyces cerevisiaeWDCM 00058GoodWellemia sebiWDCM 00182GoodEscherichia coliWDCM 00013InhibitedBacillus subtilisWDCM 00003InhibitedTABLE OF SYMBOLS Batch code Do not reuse Manufacturer Use by Fragile, handle withcare Catalogue number Temperature limitation Contains sufficientfor <n> tests Caution, consult instructions for use LIOFILCHEM S.r.l.Via Scozia, Zona
- Performance: testing of culture media. 2.ISO 21527-2:2008. Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of yeasts and moulds. Part 2: Colony Count
Storage, hazards, and disposal
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for professional use only and must be used by properly trained operators.
- Storage / shelf life: AND TRANSPORT CONDITIONSThe powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.REFERENCES1.EN ISO 11133:2014. Microbiology of food, animal feed and water
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classif ication in any hazard class in accordance to Regula tion (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610238
- Catalog/document code matched: 610238, 21527
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/dichloran-glycerol-dg18-agar-base-for-microbiology-lab-testing