Laboratory use profile
Liofilchem Campylobacter KARMALI Agar Base Culture Media 610200 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is campylobacter Karmali Agar Base is a blood-free, charcoal-based medium used with supplements for the selective isolation of Campylobacter jejuni and Campylobacter coli from feces and other materials.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Use case and sample types
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Decision criteria after incubation
The typical colonies of Campylobacter are small, greyish and flat with irregular edges. Colonies have a tendency to spread, especially when initially isolated from fresh clinical specimens.
Method logic
Peptospecial provides amino acids, nitrogen, carbon, minerals, vitamins and other nutrients which support the growth of microorganisms. Corn starch is a source of energy. Activated charcoal helps to neutralize the toxic metabolic products formed in the medium. Sodium chloride maintains the osmotic balance of the medium. Agar is the solidifying agent.Supplementation with Campylobacter Karmali Supplement (ref. 81036), containing cefoperazone, vancomycin, cycloheximide, hemin and sodium pyruvate, inhibits the contaminant microbial flora in fecal specimens improving, at the same time, the recovery of Campylobacter spp.
Preparation and execution notes
- Preparation: Suspend 48.0 g of powder in 1 liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Sterilize at 121°C for 15 minutes. Cool up to 45-50°C. Aseptically, add rehydrated content of 2 vials (10 ml) of Campylobacter Karmali Supplement. Pour in Petri dishes.
- Procedure: Inoculate the plates by streaking directly the sample onto the agar surface. Incubate under microaerobic atmosphere (containing approximately 5% O2, 10% CO2 and 85% N2) at 42°C for a minimum of 48 hours.
Formula, QC, and performance
- Formula / composition: (g/l)Peptospecial23.0Starch 1.0Activated Charcoal 4.0Sodium Chloride 5.0Agar15.0Final pH 7.4 ± 0.2 at 25°C
Risk controls for routine use
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for in vitro diagnostic use only and must be used by properly trained operators.
- Storage / shelf life: AND TRANSPORT CONDITIONSThe powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610200
- Catalog/document code matched: 610200
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/campylobacter-karmali-agar-base-for-microbiology-lab-testing