Investigation 9: Biotechnology - Restriction Enzyme Analysis of DNA Biotech Education Kit

EDVOTEK Biotechnology - NEW & IMPROVED - Now for 10 Lab Groups!

Analysis of Eco RI Cleavage Patterns of Lambda DNA

This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bateriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.

• Introduce restriction enzymes and their ability to digest DNA at specific nucleotide sequences
• Understand how electrophoresis separates differently-sized molecules
• Perform agarose gel electrophoresis
• Load, run, analyze, compare and size lambda DNA cut with HindIII, lambda DNA cut with EcoRI, and uncut lambda DNA

Key specifications

• Brand / Manufacturer: EDVOTEK
• Manufacturer Catalog No.: AP09-112
• Product Type: Biotechnology Education Kit
• Product Category: Business & Industrial > Medical > Medical Teaching Equipment
• Application: hands-on biotechnology teaching laboratories
• Use: Professional laboratory use only unless otherwise stated by the manufacturer.

Documentation

SDS/MSDS, IFU, certificate, COA, or technical sheet availability can vary by lot and manufacturer. Confirm the latest required documentation before purchase or before use in regulated laboratory workflows.

For storage conditions, product documentation, or any additional technical details, please contact us and we will be happy to provide the available information.