Brain Heart Infusion Broth Culture Media

© Liofilchem® - Brain Heart Infusion Broth - Rev.1 / 15.05.2020

Instructions For Use

Description

Brain Heart Infusion Broth is a liquid medium used for the cultivation of fastidious and nonfastidious microorganisms, including aerobic and anaerobic bacteria, from clinical specimens, food and environmental samples. This medium is especially suited for the cultivation of coagulase-positive staphylococci for the plasma coagulase test according to ISO 6888. Brain Heart Infusion Broth is recommended by the APHA for examination of water and wastewater and by the CLSI for preparing inocula used in antimicrobial susceptibility tests.

Preparation

• Dehydrated medium Suspend 37 g of the powder in 1 liter of distilled or deionized water
• Heat to boil shaking frequently until completely dissolved
• Distribute into final containers
• Sterilize in autoclave at 121°C for 15 minutes
• TEST PROCEDURE If material is being cultured directly from a swab, insert the swab into the broth after inoculation of plated media
• For liquid specimens, transfer a loopful of the specimen into the broth medium using a sterile loop or aseptically pipette the specimen onto plated medium and into the broth
• Examine for growth after 24-72 hours of incubation
• It is recommended that liquid media for anaerobic incubation should be reduced prior to inoculation by placing tubes (with loosened caps) under anaerobic conditions for 18-24 hours
• Alternatively, the media may be reduced by bringing the media up to 100°C in a boiling waterbath
• Loosen screw caps slightly before heating, and tighten during cooling to room temperature
• To perform plasma coagulase tests, according to ISO 6888, inoculate tubes of Brain Heart Infusion Broth with selected colony from Baird Parker Agar plates (ref
• Incubate at 37 ± 1°C for 24 ± 2 hours
• Add 0.1 ml of each culture to 0.3 ml of the rabbit plasma
• Examine after 4-6 hours incubation at 37°C for clotting of the plasma
• INTERPRETING RESULTS Turbidity indicates microbial growth
• The coagulase test is considered positive if the clot volume is more than half of the original liquid volume.

Technique

• APHA (2012): Standard Methods for the Examination of Water
• American Public Health Association, American Water Works Association, Water Environment Federation, Washington, D.C
• Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - Third Edition
• CLSI document M22-A3
• Wayne, PA: Clinical and Laboratory Standards Institute, 2004
• ISO 6888-3:2003
• Microbiology of food and animal feeding stuffs − Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) −Detection and MPN

Specifications

• Storage: 2
• Shelf life: Medio deshidratado: 4 años